LOCUS       sequence-102160-         604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (2x35S) for direct cloning of artificial 
            microRNAs into rice MIR390 precursor. To use for expressing amiRNAs 
            in monocots..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Carbonell A, Fahlgren N, Mitchell S, Cox KL Jr, Mockler TC, 
            Carrington JC
  TITLE     Highly Specific Gene Silencing in a Monocot Species by Artificial 
            MicroRNAs Derived From Chimeric MIRNA Precursors.
  JOURNAL   Plant J. 2015 Mar 24. doi: 10.1111/tpj.12835.
  PUBMED    25809382
REFERENCE   2  (bases 1 to 604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2015 Mar 24. doi: 10.1111/tpj.12835."
COMMENT     Sequence Label: pMDC32B-OsMIR390-B/c
FEATURES             Location/Qualifiers
     source          1..604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(172..196)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      260..512
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(529..545)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(529..545)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(542..564)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    553..569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
       61 gaatataaat gtcaggctcc cttatacaca gccagtctgc acctcgacgg tctcacatgg
      121 tttgttctta ccacacgacc aattaaatcg agctcaaggg tgggcgcgcc gacccagctt
      181 tcttgtacaa agtggttcga taattcttaa ttaactagtt ctagagcggc cgccaccgcg
      241 gtggagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
      301 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
      361 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
      421 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
      481 atcgcgcgcg gtgtcatcta tgttactaga tcgggaattc gtaatcatgt catagctgtt
      541 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
      601 gtgt
//
