LOCUS       sequence-102170-         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (2x35S) for direct cloning of artificial 
            microRNAs into rice MIR390 precursor. To use for expressing amiRNAs 
            in monocots..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Carbonell A, Fahlgren N, Mitchell S, Cox KL Jr, Mockler TC, 
            Carrington JC
  TITLE     Highly Specific Gene Silencing in a Monocot Species by Artificial 
            MicroRNAs Derived From Chimeric MIRNA Precursors.
  JOURNAL   Plant J. 2015 Mar 24. doi: 10.1111/tpj.12835.
  PUBMED    25809382
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2015 Mar 24. doi: 10.1111/tpj.12835."
COMMENT     Sequence Label: pMDC123SB-OsMIR390-B/c
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(79..85,86..103,104..109))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(194..218)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(278..622)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(314..334)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgacggct
       61 cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc
      121 ctaatggtct ctcaaggatt gttccatacc cttcctcaaa acatctcgag ctcggtgaag
      181 ggggcggccg cggagcctgc ttttttgtac aaacttgttt gatagcttgg cgcgcctcga
      241 gggggggccc ggtacccggg gatcctctag agtcgaggtc ctctccaaat gaaatgaact
      301 tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc
      361 agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
      421 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttc
      481 aacgatggcc tttcctttat cgcaatgatg gcatttgtag gagccacctt ccttttccac
      541 tatcttcaca ataaagtgac agatagctgg gcaatggaat ccgaggaggt ttccggatat
      601 taccctttgt tgaaaagtct caattgccct ttggtcttct gagactgtat ctttgatatt
      661 tttggagtag acaagtgtgt cgtgctccac catg
//
