LOCUS       sequence-102210-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of rat Cx43 fused to mAPPLE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Johnson KE, Mitra S, Katoch P, Kelsey LS, Johnson KR, Mehta PP
  TITLE     Phosphorylation on Ser-279 and Ser-282 of connexin43 regulates 
            endocytosis and gap junction assembly in pancreatic cancer cells.
  JOURNAL   Mol Biol Cell. 2013 Mar;24(6):715-33. doi: 10.1091/mbc.E12-07-0537. 
            Epub 2013 Jan 30.
  PUBMED    23363606
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E12-07-0537"; journalName: "Mol Biol Cell"; date: 
            "2013-03"; volume: "24"; issue: "6"; pages: "715-33"
COMMENT     Sequence Label: LXSN-Cx43-mAPPLE
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(268..287)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      697..706
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctcgtccat gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg
       61 tgtagtcctc gttgtgggac acgatgtcca acttgatgtc gacgatgtag gcgccgggca
      121 gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc ggcggcgtag tggccgccgt
      181 ccttcagctt cagcctcttc ttgatctcgc tcttcagggc gccgtcctcg gggtacatcc
      241 gctcctcgga ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga
      301 agttggtgcc gcgcagcttc accttgtaga tgaacacgcc gtcctgcagg gaggagtcct
      361 ggttaacgtg aataatgccg ccgtcctcga agttcatcac gcgctcccac ctgaagccct
      421 cggggaagga cagcttgaag tagtcgggga tgtcggctgg gtgcttaatg tagaccttgg
      481 agccgtacat gaactgaggg gacaggatgt cccaggcgaa gggcaggggg ccacccttgg
      541 tcaccttcag cttagcggtc tgaaaggcct cgtaggggcg gccctcgccc tcgccctcga
      601 tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc atgaactcct
      661 tgatgatggc catgttattc tcctcgccct tgctcaccat ggtggcgacc ggtggatccg
      721 caatctccag gtcatcaggc cgaggcctgc tgctggcgcg gctgctggct ctgct
//
