LOCUS       sequence-102215-        4976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circuit: NOR gate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Nielsen AA, Voigt CA
  TITLE     Multi-input CRISPR/Cas genetic circuits that interface host 
            regulatory networks.
  JOURNAL   Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
  PUBMED    25422271
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-24"; volume: "10:763. doi"; pages: " 
            10.15252/msb.20145735"
COMMENT     Sequence Label: pAN-NOR
FEATURES             Location/Qualifiers
     source          1..4976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(169..757)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(249..268)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(928..1719,1720..1788))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1551..1570
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1789..1893)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1974..2008
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     terminator      2752..2823
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2839..2866
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_RNA        2963..3038
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    3262..3283
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(3284..4162)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4189..4473
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4396..4415
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     misc_RNA        4494..4569
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4590..4619
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4705..4722)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4875..4921
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(join(4974..4976,1..15))
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
       61 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      121 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct
      181 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      241 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      301 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      361 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      421 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      481 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      541 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      601 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      661 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
      721 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
      781 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
      841 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
      901 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
      961 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     1021 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     1081 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     1141 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     1201 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     1261 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     1321 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     1381 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     1441 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     1501 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     1561 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     1621 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     1681 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     1741 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     1801 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     1861 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     1921 cgtctaagaa accattatta tcatgacatt aaccagttga taagtcccta acttttacag
     1981 ctagctcagt cctaggtatt atgctagcct gaagtacgtc tgagcgtgat acccgctcac
     2041 tgaagatggc ccggtagggc cgaaacgtac ctctacaaat aattttgttt aactatggac
     2101 tatgtttgaa agggagaaat actagatggc acgtaccccg agccgtagca gcattggtag
     2161 cctgcgtagt ccgcataccc ataaagcaat tctgaccagc accattgaaa tcctgaaaga
     2221 atgtggttat agcggtctga gcattgaaag cgttgcacgt cgtgccggtg caagcaaacc
     2281 gaccatttat cgttggtgga ccaataaagc agcactgatt gccgaagtgt atgaaaatga
     2341 aagcgaacag gtgcgtaaat ttccggatct gggtagcttt aaagccgatc tggattttct
     2401 gctgcgtaat ctgtggaaag tttggcgtga aaccatttgt ggtgaagcat ttcgttgtgt
     2461 tattgcagaa gcacagctgg accctgcaac cctgacccag ctgaaagatc agtttatgga
     2521 acgtcgtcgt gagatgccga aaaaactggt tgaaaatgcc attagcaatg gtgaactgcc
     2581 gaaagatacc aatcgtgaac tgctgctgga tatgattttt ggtttttgtt ggtatcgcct
     2641 gctgaccgaa cagctgaccg ttgaacagga tattgaagaa tttaccttcc tgctgattaa
     2701 tggtgtttgt ccgggtacac agcgttaact agggcccata cccccaggca tcaaataaaa
     2761 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2821 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatacgcgac
     2881 gtacggtgga atctgattcg ttaccaattg acatgatacg aaacgtaccg tatcgttaag
     2941 gtataatacc cgtgtgaccc gtgttttaga gctagaaata gcaagttaaa ataaggctag
     3001 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttctcgg taccaaagac
     3061 gaacaataag acgctgaaaa gcgtcttttt tcgttttggt ccggagctcg gtaccaaatt
     3121 ccagaaaaga ggcctcccga aaggggggcc ttttttcgtt ttggtcctac gttcagccaa
     3181 aaaacttaag accgccggtc ttgtccacta ccttgcagta atgcggtgga caggatcggc
     3241 ggttttcttt tctcttctca agaattcgcg gccgcttcta gagttatgac aacttgacgg
     3301 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     3361 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     3421 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct
     3481 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     3541 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     3601 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt
     3661 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     3721 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     3781 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa
     3841 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     3901 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     3961 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     4021 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     4081 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg
     4141 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     4201 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca
     4261 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     4321 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     4381 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     4441 gctttttatc gcaactctct actgtttctc catataatac ccgtgtgacc cgtgttttag
     4501 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
     4561 agtcggtgct ttttttgaag cttgggcccg aacaaaaact catctcagaa gaggatctga
     4621 atagtgccgt cgaccatcat catcatcatt gagtttaaac ggtctccagc ttggctgttt
     4681 tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct
     4741 gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa
     4801 ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg
     4861 gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     4921 tctgttgttt gtcggtgaac tggatcctta ctcgagtcta gactgcaggc ttcctc
//
