LOCUS       sequence-102316-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Middle Gateway Entry Vector Containing QF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Subedi A, Macurak M, Gee ST, Monge E, Goll MG, Potter CJ, Parsons 
            MJ, Halpern ME
  TITLE     Adoption of the Q transcriptional regulatory system for zebrafish 
            transgenesis.
  JOURNAL   Methods. 2014 Apr 1;66(3):433-40. doi: 10.1016/j.ymeth.2013.06.012. 
            Epub 2013 Jun 20.
  PUBMED    23792917
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2013.06.012"; journalName: "Methods"; date: 
            "2014-04-1- 1"; volume: "66"; issue: "3"; pages: "433-40"
COMMENT     Sequence Label: pME-QF
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..101
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    114..248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(138..157)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     192..211
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(520..540)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          670..735
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 ggcaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca
       61 atgctttctt ataatgccaa ctttgtacaa gaaagctggg tggccgcaga tccgatccag
      121 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
      181 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
      241 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
      301 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctctagt
      361 caaggcacta tacatcaaat atttttccat aattttcttg tatagcagtg cagctttttc
      421 ctttgtggtg taaatagcaa agcaagcaag agttctatta ctaaacacag catgactcaa
      481 aaaacttagc aattctgaag gaaagtcctt ggggtcttct acctttctct tcttttttgg
      541 aggagtagaa tgttgagagt cagcagtagc ctcatcatca ctagatggca tttcttctga
      601 gcaaaacagg ttttcctcat taaaggcatt ccaccactgc tcccattcat cagttccata
      661 ggttggaatc taaaatacac aaacaattag aatcagtagt ttaacacatt atacacttaa
      721 aaattttata tttaccttag agctttaaat ctctgtaggt agtttgtcca attatgtcac
      781 accacagaag taaggttcct tcacaaagat cctctagacg tcctattgct catacgtgtt
      841 gatatcgcta aaattacacc ctgggacaaa cccgaggttc gtcatgaac
//
