LOCUS       sequence-102457-        1963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with MCS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_MCS2
FEATURES             Location/Qualifiers
     source          1..1963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        71..175
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(176..244,245..1036)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(394..413)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1207..1795
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1696..1715
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgaggaatt caaagatctt cggtaccgtc gaccaattcg
       61 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      121 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      181 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      241 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
      301 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
      361 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
      421 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
      481 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
      541 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
      601 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
      661 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
      721 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
      781 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
      841 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
      901 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
      961 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1021 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1081 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1141 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1201 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1261 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1321 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1381 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1441 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1501 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1561 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1621 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1681 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1741 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1801 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1861 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1921 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agc
//
