LOCUS       sequence-102476-        2147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with minPGK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_minPGK
FEATURES             Location/Qualifiers
     source          1..2147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     162..181
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        255..359
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(360..428,429..1220)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(578..597)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1391..1979
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1880..1899
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagctttg gggcagcggc caatagcagc tttgctcctt
       61 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
      121 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
      181 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cgtcgaccaa
      241 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      301 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      361 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      421 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
      481 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
      541 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
      601 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      661 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      721 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
      781 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
      841 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
      901 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
      961 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1021 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     1081 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1141 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1201 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1261 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1321 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1381 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1441 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1501 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1561 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1621 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1681 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1741 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1801 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1861 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1921 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1981 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2041 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     2101 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagc
//
