LOCUS       sequence-102483-        2357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with Tet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_Tet
FEATURES             Location/Qualifiers
     source          1..2357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(43..61,62..84,85..103,104..126,127..145,146..168,
                     169..187,188..210,211..229,230..252,253..271,272..294,
                     295..313)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     340..364
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     rep_origin      complement(621..1209)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(701..720)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1380..2171,2172..2240))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2003..2022
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2241..2345)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aattacgcgt aactcgaggg taccgagctc gactttcact tttctctatc actgataggg
       61 agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt
      121 cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg
      181 atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc
      241 gactttcact tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta
      301 tcactgatag ggagtggtaa actcgaccta tataagcaga gctcgtttag tgaaccgtca
      361 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      421 cagcctccgc ggccccgaat tcgtcgacca tggctcactg actcgctgcg ctcggtcgtt
      481 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      541 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      601 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      661 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      721 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      781 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      841 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      901 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      961 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1021 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1081 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1141 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1201 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1261 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1321 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     1381 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1441 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1501 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     1561 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1621 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     1681 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     1741 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     1801 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     1861 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     1921 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     1981 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     2041 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     2101 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     2161 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     2221 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2281 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2341 ccgcgcacat ttccccg
//
