LOCUS       sequence-102747-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template for generating gene 60 mRNA, coding gap deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Todd GC, Walter NG
  TITLE     Secondary structure of bacteriophage T4 gene 60 mRNA: implications 
            for translational bypassing.
  JOURNAL   RNA. 2013 May;19(5):685-700. doi: 10.1261/rna.037291.112. Epub 2013 
            Mar 14.
  PUBMED    23492219
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.037291.112"; journalName: "RNA"; date: "2013-05"; 
            volume: "19"; issue: "5"; pages: "685-700"
COMMENT     Sequence Label: pUC19 gene 60 delta gap
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(628..644)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(628..644)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(641..663)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    652..668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(676..682,683..700,701..706))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    721..742
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(859..876)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cctctagata atacgactca ctataggagc atttaatgaa tttaaacgcg gttggattcc
       61 tgatgaaaag ttctatgagg tgtataatga aatttgtaaa aattgattct tctagcgttg
      121 atatgaaaaa atataaattg cagaacaatg ttcgtcgttc tattaaatcc tcttcaatga
      181 actatgcgaa tgtcgctatt atgacagacg cagatcacga tggattaggt tctatttatc
      241 cttctctgct cggatttttt agtaattggc cagaattgtt tgagcaagga cgaattcgct
      301 ttgtcaaaac tcctgtaatc atcgctcagg tcggtaaaaa acaagaatgg ttttatacag
      361 tcgctgaata tgagagtgcc aaagatgctc tacctaaaca tagcatccgt tatattaagg
      421 gacttggctc tttggaaaaa tctgaatatc gtgagatgat tcaaaaccca gtatatgatg
      481 ttgttaaact tcctgagaac tggaaagagc tttttgaaat gctcatggga gataatgctg
      541 accttcgtaa agaatggatg agccagtagt ttactttacc acaaggatgt ggtataatta
      601 attgggcaaa agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      661 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      721 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      781 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      841 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      901 agcggtatca gctcactcaa
//
