LOCUS       sequence-102753-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template for generating gene 60 mRNA truncated two codons after 
            coding gap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Todd GC, Walter NG
  TITLE     Secondary structure of bacteriophage T4 gene 60 mRNA: implications 
            for translational bypassing.
  JOURNAL   RNA. 2013 May;19(5):685-700. doi: 10.1261/rna.037291.112. Epub 2013 
            Mar 14.
  PUBMED    23492219
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.037291.112"; journalName: "RNA"; date: "2013-05"; 
            volume: "19"; issue: "5"; pages: "685-700"
COMMENT     Sequence Label: pUC19 gene 60 thru gap
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(342..358)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(342..358)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(355..377)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    366..382
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(390..396,397..414,415..420))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    435..456
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(573..590)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cctctagata atacgactca ctataggagc atttaatgaa tttaaacgcg gttggattcc
       61 tgatgaaaag ttctatgagg tgtataatga aatttgtaaa aattgattct tctagcgttg
      121 atatgaaaaa atataaattg cagaacaatg ttcgtcgttc tattaaatcc tcttcaatga
      181 actatgcgaa tgtcgctatt atgacagacg cagatcacga tggatagcct tcgggctatc
      241 tatagaaata cctcataatt aagagattat tggattaggt tagtttactt taccacaagg
      301 atgtggtata attaattggg caaaagcttg gcgtaatcat ggtcatagct gtttcctgtg
      361 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      421 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      481 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      541 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      601 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      661 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      721 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaa
//
