LOCUS       sequence-102762-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inserts a puromycin resistance gene and a VSG117 gene into the 
            VSG221 expression site of T.brucei downstream of the expression site
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Smith TK, Vasileva N, Gluenz E, Terry S, Portman N, Kramer S, 
            Carrington M, Michaeli S, Gull K, Rudenko G
  TITLE     Blocking variant surface glycoprotein synthesis in Trypanosoma 
            brucei triggers a general arrest in translation initiation.
  JOURNAL   PLoS One. 2009 Oct 26;4(10):e7532. doi: 
            10.1371/journal.pone.0007532.
  PUBMED    19855834
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-10-26"; volume: "4(10):e7532. doi"; pages: " 
            10.1371/journal.pone.0007532"
COMMENT     Sequence Label: p221- purVSG117UTR
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcgaggtgct tcatctgctg gtcgtcttca aggccttgtt tacgttctgg gagacacttg
       61 cacttcgagg tccgattgtt tattgggttt tgaagtaggt ttccagatga aatcattttc
      121 tccaattttg ttgccaccac caaccttccc aacaacagca aactgtaaac caaaaatcac
      181 ccaaaagcgc cgctgcatct gcaataccca cccatacaca gggcagctca cctccaccct
      241 ccaaattcat tgcaggtggc atttatgaaa gcgaagcagc aaagcccacg cgatttttat
      301 agtatcaaat caccagtgtt gattcgtcgt tattttcctg ttctcccgaa gcatcttggc
      361 gatgcagcat catcctgcgt cgttcccacg catatcaacc atcaataatt ttcagtatat
      421 ttgtacggat tcccgagcac agccaccgtc tcacgacgat tttactgtct tgcttcctcc
      481 tccccagcac ccctcgcggg cacttgtcaa acacctcagc actctgcgtt attaccccac
      541 ccacaccgtt tgggccattt acacacgctc tctacacgct tcaagccttc gccatcctcg
      601 cgcacgatgt ccagttacca tttccctctt cacgtttttc ctaaccctca caatctccga
      661 tcatgctaat tccaccattt cctctaatcc tttcactctt ccgttgca
//
