LOCUS       sequence-102766-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For replacement of VSG221 with a blasticidin resistence gene in the 
            VSG221 expression site in T.brucei..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  TITLE     pBSVSG221KOblast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBSVSG221KOblast
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(777..795)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tatcgaattc ctgcagcccg ggggatcctg tcgcgtagga ataactacaa tcttcaagag
       61 taattaaaat attgtgggaa aacatgcaaa ttttcgtctt ctggaaatta agctggttct
      121 gtagcatcag cggcaaacac ggccgtttgt cttgccatta tgttttggca gagcgttgct
      181 cctatatact agatctttta cgtatagaat gcttgtgtgt cttaacatat gtatagcggt
      241 gtccaaatgc aatctaaccg gctcttcgtc tccgaattct tttattgctt tttcttgaac
      301 taaatgctac agaatcacgt aaggaggatg aaattgtgct tgtaaatgga aactatttac
      361 tggcatatta ctgctagctt gaatcgacat gaaatattct aagtgttctc atttttaata
      421 aaataaatct gtcatttaaa atgcattggc acactttccg ctgccttcat tttagataat
      481 cagaaaaacc tgagcgccat ctaatagttt agaaaattca cttgtgtgta cgctccatta
      541 atgttaaacg tttccacact atcctaaggt gcaataaaag tttttctttt tatttccttt
      601 atttgtacat tctctcattt ccttttattc tttctaaatt ggcactgctt ttaacagttt
      661 ctgcaagacg tggtgcaatc atcagctgtt tggcttgcgt ctgcctgtga cgagaggtag
      721 ttacatatgc tgcatctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt
      781 tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctg
//
