LOCUS       sequence-102799-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector encoding long isoform of human DIXDC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Goodwin JM, Svensson RU, Lou HJ, Winslow MM, Turk BE, Shaw RJ
  TITLE     An AMPK-independent signaling pathway downstream of the LKB1 tumor 
            suppressor controls Snail1 and metastatic potential.
  JOURNAL   Mol Cell. 2014 Aug 7;55(3):436-50. doi: 
            10.1016/j.molcel.2014.06.021. Epub 2014 Jul 17.
  PUBMED    25042806
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.06.021"; journalName: "Mol Cell"; date: 
            "2014-08-7- 7"; volume: "55"; issue: "3"; pages: "436-50"
COMMENT     Sequence Label: pcDNA3-FLAG-hDIXDC1 WT
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    88..112
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             121..144
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagctg agcatcaaca agtttgtaca aaaaagcagg ctccaccatg
      121 gattacaagg acgatgacga caaactcgga tccatgggaa ccaattcagt agacatgcta
      181 gcctgcctga cccgagggaa cttactggac gtcctgcagg agggcttcaa tgagcaacag
      241 ctgcaggcct atgtggcctg ggtgaatgca cagctgaaga agaggccagc agtgaagcct
      301 gtgcaggacc tgcgacaaga tctccgggat ggggtgatcc tggcatatct catcgagatt
      361 gttgcaggag aaaagctgag tggggtacag ctgagtcccg gtaaccaaca ggagatgaag
      421 aataatgtgg agaaagtgct acagtttgtg gcctctaaaa agattcgtat gcaccagact
      481 tcggctaaag atattgtgga tggaaacctg aagtctatca tgaggctggt ccttgccttg
      541 gcagctcatt tcaaacctgg ctccagcagg acggtgaacc aaggacggga ctccagagcc
      601 cctctgcaaa gtcaccgacc acactgtgcc actgctgttg cccagggagc agctgctgct
      661 ctggccgatg tgtgtcatga catgtcccga tcaggacggg atgtctttcg atatagacag
      721 aggaacagca gcatggatga ggaaattgag aatccatact ggagtgtgcg ggccctagtg
      781 cagcagtacg aagggcaaca aaggtccccg tctgaatcca gctgctccag cctgacttca
      841 cccagtccaa tccacagtgc aa
//
