LOCUS       sequence-102821-         448 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mRNA synthesis or expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Matoba S, Liu Y, Lu F, Iwabuchi KA, Shen L, Inoue A, Zhang Y
  TITLE     Embryonic Development following Somatic Cell Nuclear Transfer 
            Impeded by Persisting Histone Methylation.
  JOURNAL   Cell. 2014 Nov 6;159(4):884-95. doi: 10.1016/j.cell.2014.09.055. 
            Epub 2014 Oct 30.
  PUBMED    25417163
REFERENCE   2  (bases 1 to 448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.09.055"; journalName: "Cell"; date: "2014-11-6-
            6"; volume: "159"; issue: "4"; pages: "884-95"
COMMENT     Sequence Label: pcDNA-flag-mKdm4d-polyA
FEATURES             Location/Qualifiers
     source          1..448
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    28..252
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     241..258
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(281..298)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(314..343)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cccagctggt tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc
       61 tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat
      121 gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag
      181 tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac
      241 tagaaggcac agtcgaggct gatcagcggg tttaaactca atggtgatgg tgatgatgac
      301 cggtatgcat attcagatcc tcttctgaga tgagtttttg ttcgaagggc cctctagact
      361 cgaggtacgc cacagcgtat gatgcgtatt tttttttttt tttttttttt tttttttttt
      421 tttttttttt tttttttttt tttttttt
//
