LOCUS       sequence-102923-        1161 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of cdc42 wt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Coso OA, Chiariello M, Yu JC, Teramoto H, Crespo P, Xu N, Miki T, 
            Gutkind JS
  TITLE     The small GTP-binding proteins Rac1 and Cdc42 regulate the activity 
            of the JNK/SAPK signaling pathway.
  JOURNAL   Cell. 1995 Jun 30;81(7):1137-46.
  PUBMED    7600581
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1995-06-30"; volume: "81(7)"; pages: "1137-46"
COMMENT     Sequence Label: pCDNAIIIB cdc42 WT
FEATURES             Location/Qualifiers
     source          1..1161
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2..19
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(73..648)
                     /codon_start=1
                     /product="human Rho family small GTPase Cdc42"
                     /label=Cdc42
                     /translation="MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVM
                     IGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH
                     CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK
                     GLKNVFDEAILAALEPPEPKKSRRCVLL"
     primer_bind     complement(685..704)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(686..704)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(749..952)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(778..798)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 catttaggtg acactataga atagggccct ctagatgcat gctcgagcgg ccgctcgagt
       61 cgacccggga attcatagca gcacacacct gcggctcttc ttcggttctg gaggctccag
      121 ggcagccaat attgcttcgt caaatacatt ctttaggcct ttctgtgtaa gtgcagaaca
      181 ctccacatac ttgacagcct tcaggtcacg ggccagcttt tcagcagtct ctggagtgat
      241 aggcttctgt ttgttcttgg caagtttctc aatagtagag gggtcatctc tgagatcaat
      301 ttgagtccca acaagcaaga aaggagtctt tggacagtgg tgagttatct caggcaccca
      361 cttttctttc acgttttcaa atgaagatgg agagaccact gaaaaacaga ctagaaatac
      421 atctgtttgt ggataactca gcggtcgtaa tctgtcataa tcctcttgcc ctgcagtatc
      481 aaaaagtcca agagtatatg gttctccacc aatcataact gtgactgcat agttgtcaaa
      541 aacagtcggt acatattccg atggaaattt gtttgttgtg taggatatca ggagacatgt
      601 tttaccaaca gcaccatcgc ccacaacaac acacttaatt gtctgcatgg tggcggatcc
      661 gagctcggta ccaagcttgg gtctccctat agtgagtcgt attaatttcg ataagccagt
      721 aagcagtggg ttctctagtt agccagagag ctctgcttat atagacctcc caccgtacac
      781 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
      841 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
      901 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
      961 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
     1021 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     1081 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     1141 gtcaatggaa agtccctatt g
//
