LOCUS       sequence-102927-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of rac1 constitutively active mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Coso OA, Chiariello M, Yu JC, Teramoto H, Crespo P, Xu N, Miki T, 
            Gutkind JS
  TITLE     The small GTP-binding proteins Rac1 and Cdc42 regulate the activity 
            of the JNK/SAPK signaling pathway.
  JOURNAL   Cell. 1995 Jun 30;81(7):1137-46.
  PUBMED    7600581
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1995-06-30"; volume: "81(7)"; pages: "1137-46"
COMMENT     Sequence Label: pCDNAIIIB rac1 QL
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(117..296,297..299,300..695)
                     /codon_start=1
                     /product="constitutively active mutant of human Rac family 
                     small GTPase 1"
                     /label=Rac1 (Q61L)
                     /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGLEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     promoter        complement(750..768)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(751..768)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(788..805)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    794..1018
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccga gctcggatcc gccaccatgc
      121 aggccatcaa gtgtgtggtg gtgggagacg gagctgtagg taaaacttgc ctactgatca
      181 gttacacaac caatgcattt cctggagaat atatccctac tgtctttgac aattattctg
      241 ccaatgttat ggtagatgga aaaccggtga atctgggctt atgggataca gctggactcg
      301 aggattatga cagattacgc cccctatcct atccgcaaac agatgtgttc ttaatttgct
      361 tttcccttgt gagtcctgca tcatttgaaa atgtccgtgc aaagtggtat cctgaggtgc
      421 ggcaccactg tcccaacact cccatcatcc tagtgggaac taaacttgat cttagggatg
      481 ataaagacac gatcgagaaa ctgaaggaga agaagctgac tcccatcacc tatccgcagg
      541 gtctagccat ggctaaggag attggtgctg taaaatacct ggagtgctcg gcgctcacac
      601 agcgaggcct caagacagtg tttgacgaag cgatccgagc agtcctctgc ccgcctcccg
      661 tgaagaagag gaagagaaaa tgcctgctgt tgtaagaatt cccgggtcga ctcgagcggc
      721 cgctcgagca tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc
      781 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
      841 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
      901 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
      961 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     1021 tctgaggcgg aaagaaccag ctggggctct agggggtat
//
