LOCUS       sequence-102954-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based biosensor for monitoring JNK activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Fosbrink M, Aye-Han NN, Cheong R, Levchenko A, Zhang J
  TITLE     Visualization of JNK activity dynamics with a genetically encoded 
            fluorescent biosensor.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5459-64. doi: 
            10.1073/pnas.0909671107. Epub 2010 Mar 8.
  PUBMED    20212108
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0909671107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-03-23- 23"; volume: "107"; issue: "12"; pages: "5459-64"
COMMENT     Sequence Label: pcDNA3-JNKAR1
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(87..89,90..92,93..605)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(132..153)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(393..412)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     740..761
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatcccatgg tgagcaaggg cgaggagctg ttcaccgggg
      121 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaggttc agcgtgtccg
      181 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      241 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc gtgcagtgct
      301 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      361 gctacgtcca ggagcgtacc atcttcttca aggacgacgg caactacaag acccgcgccg
      421 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      481 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc cacaacgtct
      541 atatcaccgc cgacaagcag aagaacggca tcaaggccca cttcaagatc cgccacaaca
      601 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      661 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc
      721 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc cgcatgcata
      781 a
//
