LOCUS       sequence-102986-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the yeast Prp2 protein for overexpression and purification 
            using a C-terminal hexahistidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Krishnan R, Blanco MR, Kahlscheuer ML, Abelson J, Guthrie C, Walter 
            NG
  TITLE     Biased Brownian ratcheting leads to pre-mRNA remodeling and capture 
            prior to first-step splicing.
  JOURNAL   Nat Struct Mol Biol. 2013 Dec;20(12):1450-7. doi: 10.1038/nsmb.2704.
            Epub 2013 Nov 17.
  PUBMED    24240612
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2704";
            journalName: "Nat Struct Mol Biol"; date: "2013-12"; volume: "20"; 
            issue: "12"; pages: "1450-7"
COMMENT     Sequence Label: Prp2-pRSETA
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(52..72)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttgttagcag ccggatcaag cttcgaattc ctaatgatga tgatgatgat gaccttgaaa
       61 ataaagattt tcggcgcccc gcctccccct atttgtttta tcgtcaatta aatctttaaa
      121 gatttgtgga accatgtcta tcaaccattc ttccttaggg ataactagac agtccctgat
      181 aaattccttc gacgttagca tcaattgttg ataaagcaca tatttgcttg gtctctgcgc
      241 cttttcctta tgattaacga aaagtatact ggtaggatga acactaacgt tcagcccacc
      301 tgatgaccta cccatggttt gatagccggt ggggcctaat tgtacaatat tcatcggaaa
      361 cccgctgatg aagcatcgag ttattctagc atttatgtag cccgctatat tgcctatctt
      421 catcctggct tggtcgttct tttcaactaa tccaaccttt tcactgcacc tgaataattg
      481 gtttctaata tttcttaccc ttaacatcgt tttgaattga attttgtggt cttgacacca
      541 acttctggaa aatttagagt ttctccattg gttgaagatt tccaaataca atatgtgatc
      601 actttccact tcgcttaaca cacttgccgc agcatctctt ttttgaccaa taaataatga
      661 aggtgtttca tgtagcatcg aaactatggt caaacactct tctagtacac cttgacattg
      721 ttcgtgcgta gcagctgtat aaagcacttt tgcaaattca ggttcacaa
//
