LOCUS       sequence-103087-         576 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLtetO-1:DsrA, pCon:ompC::T7noRBS(900bps), pLlacO-1:rpoS::gfp
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: pHL 1410
FEATURES             Location/Qualifiers
     source          1..576
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    177..195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(262..289)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(381..467)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cctgtacgat taaagcttag cgtctctgaa gtgaatcgtt gaatgcacaa taaaaaaatc
       61 ccgaccctga gggggtcggg atgaaacttg cttaagcaag aagcacttaa aaaattcgtt
      121 acaccaggaa atctgatgtg ttcccggagt atctctatca ctgataggga tgtcaatctc
      181 tatcactgat agggaggatc ctgagcggat acatatttga atgtatttag aaaaataaac
      241 aaaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttaatttga
      301 tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcgcaacg
      361 ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
      421 ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctggcagtt
      481 ccctactctc gcatggggag accccacact accatcggcg ctacggcgtt tcacttctga
      541 gttcggcatg gggtcaggtg ggaccaccgc gctact
//
