LOCUS       sequence-103160-         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Mouse p53 (residues 1-87) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Daughdrill GW, Borcherds WM, Wu H
  TITLE     Disorder predictors also predict backbone dynamics for a family of 
            disordered proteins.
  JOURNAL   PLoS One. 2011;6(12):e29207. doi: 10.1371/journal.pone.0029207. Epub
            2011 Dec 15.
  PUBMED    22195023
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e29207. doi"; pages: " 
            10.1371/journal.pone.0029207. Epub 2011 Dec 15"
COMMENT     Sequence Label: pET28-p53 (Mouse 1-87)
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             62..79
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             356..373
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(426..444)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      440..487
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(611..630)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aactttaaga aggagatata ccatgggcag cagccatcat catcatcatc acagcagcgg
       61 cctggtgccg cgcggcagcc atatgatgac cgcaatggaa gaaagccaat ccgatatttc
      121 ccttgaactg cctttatcac aagaaacatt ctctggttta tggaaattac tgcctcccga
      181 agatatttta cctagccctc actgtatgga cgatttatta ttaccccaag atgttgaaga
      241 attttttgaa ggtccctctg aagccttacg tgtctctggc gcccctgctg cacaagatcc
      301 agtcaccgaa accccaggtc ccgtcgcccc cgcccccgcc accccctaac tcgagcacca
      361 ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      421 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
      481 ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc
      541 gcattaagcg nggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      601 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cgg
//
