LOCUS       sequence-103207-         597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Dog p53 (residues 1-77) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Daughdrill GW, Borcherds WM, Wu H
  TITLE     Disorder predictors also predict backbone dynamics for a family of 
            disordered proteins.
  JOURNAL   PLoS One. 2011;6(12):e29207. doi: 10.1371/journal.pone.0029207. Epub
            2011 Dec 15.
  PUBMED    22195023
REFERENCE   2  (bases 1 to 597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e29207. doi"; pages: " 
            10.1371/journal.pone.0029207. Epub 2011 Dec 15"
COMMENT     Sequence Label: pET28-p53 (Dog 1-77)
FEATURES             Location/Qualifiers
     source          1..597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             277..294
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(347..365)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      361..408
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(532..551)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cacagcagcg gcctggtgcc gcgcggcagc catatgatgg aagaatctca atcagaatta
       61 aatattgacc cccccttatc ccaagaaacc tttagtgaat tatggaacct cttacccgaa
      121 aataatgtgc tctcctccga actctgccct gctgttgatg aactgttact ccccgaatct
      181 gttgtaaatt ggttagatga agactccgac gatgccccac gtatgccagc cacctccgcc
      241 cctaccgccc caggccccgc cccaagttaa ctcgagcacc accaccacca ccactgagat
      301 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
      361 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
      421 actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      481 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      541 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatc
//
