LOCUS       sequence-103218-       12605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector for PITT landing pad knock-in into the Rosa26 
            locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12605)
  AUTHORS   Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, 
            Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
  TITLE     One-step generation of multiple transgenic mouse lines using an 
            improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
  JOURNAL   BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
  PUBMED    25887549
REFERENCE   2  (bases 1 to 12605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-04-9"; volume: "16:274. doi"; pages: " 
            10.1186/s12864-015-1432-5"
COMMENT     Sequence Label: pBDT
FEATURES             Location/Qualifiers
     source          1..12605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..643
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        625..643
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     intron          1240..1305
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1382..1954)
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     misc_feature    join(3362..3427,3428..3465)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     regulatory      3629..3638
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3635..3637,3638..3640,3641..4351)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3635..3637,3638..3640,3641..4351)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3680..3701)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3941..3960)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4288..4309
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4361..4414
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4415..5206
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4466..4485)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5076..5095
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5368..5449
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5405..5424)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(5450..5497)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    complement(5498..5531)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     misc_feature    5919..6568
                     /label=HA-R
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(10415..10434)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10416..10434)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10456..10472)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10456..10472)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10469..10491)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10480..10496
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10504..10510,10511..10528,10529..10534))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10549..10570
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10687..10704)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10858..11446)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10938..10957)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11617..12408,12409..12477))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12240..12259
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12478..12582)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
      661 atggcaattg ggccgagcaa gcttgcatgc ggccgcatct agctagagtc gatcgaccag
      721 cttctgatgg aattagaact tggcaaaaca atactgagaa tgaagtgtat gtggaacaga
      781 ggctgctgat ctcgttcttc aggctatgaa actgacacat ttggaaacca cagtacttag
      841 aaccacaaag tgggaatcaa gagaaaaaca atgatcccac gagagatcta tagatctata
      901 gatcatgagt gggaggaatg agctggccct taatttggtt ttgcttgttt aaattatgat
      961 atccaactat gaaacattat cataaagcaa tagtaaagag ccttcagtaa agagcaggca
     1021 tttatctaat cccaccccac ccccaccccc gtagctccaa tccttccatt caaaatgtag
     1081 gtactctgtt ctcacccttc ttaacaaagt atgacaggaa aaacttccat tttagtggac
     1141 atctttattg tttaatagat catcaatttc tgcatccctc gactctagtg gatctgcatt
     1201 ccaccactgc tcccattcat cagttccata ggttggaatc taaaatacac aaacaattag
     1261 aatcagtagt ttaacacatt atacacttaa aaattttata tttaccttag agctttaaat
     1321 ctctgtaggt agtttgtcca attatgtcac accacagaag taaggttcct tcacaaagag
     1381 atcgcctgac acgatttcct gcacaggctt gagccatata ctcatacatc gcatcttggc
     1441 cacgttttcc acgggtttca aaattaatct caagttctac gcttaacgct ttcgcctgtt
     1501 cccagttatt aatatattca acgctagaac tcccctcagc gaagggaagg ctgagcacta
     1561 cacgcgaagc accatcaccg aaccttttga taaactcttc cgttccgact tgctccatca
     1621 acggttcagt gagacttaaa cctaactctt tcttaatagt ttcggcatta tccactttta
     1681 gtgcgagaac cttcgtcagt cctggatacg tcactttgac cacgcctcca gcttttccag
     1741 agagcgggtt ttcattatct acagagtatc ccgcagcgtc gtatttattg tcggtactat
     1801 aaaacccttt ccaatcatcg tcataatttc cttgtgtacc agattttggc ttttgtatac
     1861 ctttttgaat ggaatctaca taaccaggtt tagtcccgtg gtacgaagaa aagttttcca
     1921 tcacaaaaga tttagaagaa tcaacaacat catcaggatc catggcgagg acctgcaggg
     1981 tcgctcggtg ttcgaggcca cacgcgtcac cttaatatgc gaagtggacc tgggaccgcg
     2041 ccgccccgac tgcatctgcg tgttcgaatt cgccaatgac aagacgctgg gcggggtttg
     2101 ctcgacattg ggtggaaaca ttccaggcct gggtggagag gctttttgct tcctcttgca
     2161 aaaccacact gctcgacatt gggtggaaac attccaggcc tgggtggaga ggctttttgc
     2221 ttcctcttga aaaccacact gctcgagggc ccttacggcc gcggcaggcc ctccgagcgt
     2281 ggtggagccg ttctgtgaga cagccgggta cgagtcgtga cgctggaagg ggcaagcggg
     2341 tggtgggcag gaatgcggtc cgccctgcag caaccggagg gggagggaga agggagcgga
     2401 aaagtctcca ccggacgcgg ccatggctcg gggggggggg cagcggagga gcgcttccgg
     2461 ccgacgtctc gtcgctgatt ggcttctttt cctcccgccg tgtgtgaaaa cacaaatggc
     2521 gtgttttggt tggcgtaagg cgcctgtcag ttaacggcag ccggagtgcg cagccgccgg
     2581 cagcctcgct ctgcccactg ggtggggcgg gaggtaggtg gggtgaggcg agctggacgt
     2641 gcgggcgcgg tcggcctctg gcggggcggg ggaggggagg gagggtcagc gaaagtagct
     2701 cgcgcgcgag cggccgccca ccctcccctt cctctggggg agtcgtttta cccgccgccg
     2761 gccgggcctc gtcgtctgat tggctctcgg ggcccagaaa actggccctt gccattggct
     2821 cgtgttcgtg caagttgagt ccatccgccg gccagcgggg gcggcgagga ggcgctccca
     2881 ggttccggcc ctcccctcgg ccccgcgccg cagagtctgg ccgcgcgccc ctgcgcaacg
     2941 tggcaggaag cgcgcgctgg gggcggggac gggcagtagg gctgagcggc tgcggggcgg
     3001 gtgcaagcac gtttccgact tgagttgcct caagaggggc gtgctgagcc agacctccat
     3061 cgcgcactcc ggggagtgga gggaaggagc gagggctcag ttgggctgtt ttggaggcag
     3121 gaagcacttg ctctcccaaa gtcgctctga gttgttatca gtaagggagc tgcagtggag
     3181 taggcgggga gaaggccgca cccttctccg gaggggggag gggagtgttg caataccttt
     3241 ctgggagttc tctgctgcct cctggcttct gaggaccgcc ctgggcctgg gagaatccct
     3301 tccccctctt ccctcgtgat ctgcaactcc agtcttgata tcaaaactct cttaaggtag
     3361 ctagggcgca gtagtccagg gtttccttga tgatgtcata cttatcctgt cccttttttt
     3421 tccacagctc gcggttgagg acaaactctt cgcggtcttt ccagtgggga tcgacggtat
     3481 cgataagctg aagttcctat tccgaagttc ctattctatc agaagtatag gaacttcgta
     3541 gtgccccaac tggggtaacc tttgagttct ctcagttggg ggcgtagaat tattcgtata
     3601 gcatacatta tacgaagtta taccggtcgc caccatggtg agcaagggcg aggagctgtt
     3661 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     3721 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     3781 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     3841 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     3901 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     3961 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     4021 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     4081 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     4141 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     4201 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     4261 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     4321 gatcactctc ggcatggacg agctgtacaa gggcagcgga gagggcagag gaagtctgct
     4381 aacatgcggt gacgtggagg agaatcccgg ccccattgaa caagatggat tgcacgcagg
     4441 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     4501 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     4561 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     4621 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     4681 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     4741 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     4801 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     4861 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     4921 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     4981 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     5041 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     5101 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     5161 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaccca gacctggcac
     5221 ccaggagagg agcaggcagg gtcagctgcg ccgcgactct agatcataat cagccatacc
     5281 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     5341 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     5401 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgcg aagttcctat
     5461 actttctaga gaatatgaac ttcggaatag gaacttcata acttcgtata ggatacttta
     5521 tacgaagtta tgaagttcct atactcctat aagaatagga acttcggaat aggaacttct
     5581 aactataacg gtcctaaggt agcgatatct tctgggcagg cttaaaggct aacctggtgt
     5641 gtgggcgttg tcctgcaggg gaattgaaca ggtgtaaaat tggagggaca agacttccca
     5701 cagattttcg gttttgtcgg gaagtttttt aataggggca aataaggaaa atgggaggat
     5761 aggtagtcat ctggggtttt atgcagcaaa actacaggtt attattgctt gtgatccgcc
     5821 tcggagtatt ttccatcgag gtagattaaa gacatgctca cccgagtttt atactctcct
     5881 gcttgagatc cttactacag tatgaaatta cagtgtcgcg agttagacta tgtaagcaga
     5941 attttaatca tttttaaaga gcccagtact tcatatccat ttctcccgct ccttctgcag
     6001 ccttatcaaa aggtatttta gaacactcat tttagcccca ttttcattta ttatactggc
     6061 ttatccaacc cctagacaga gcattggcat tttccctttc ctgatcttag aagtctgatg
     6121 actcatgaaa ccagacagat tagttacata caccacaaat cgaggctgta gctggggcct
     6181 caacactgca gttcttttat aactccttag tacacttttt gttgatcctt tgccttgatc
     6241 cttaattttc agtgtctatc acctctcccg tcaggtggtg ttccacattt gggcctattc
     6301 tcagtccagg gagttttaca acaatagatg tattgagaat ccaacctaaa gcttaacttt
     6361 ccactcccat gaatgcctct ctcctttttc tccatttata aactgagcta ttaaccatta
     6421 atggtttcca ggtggatgtc tcctccccca atattacctg atgtatctta catattgcca
     6481 ggctgatatt ttaagacatt aaaaggtata tttcattatt gagccacatg gtattgatta
     6541 ctgcttacta aaattttgtc attgtacaca tctgtaaaag gtggttcctt ttggaatgca
     6601 aagttcaggt gtttgttgtc tttcctgacc taaggtcttg tgagcttgta ttttttctat
     6661 ttaagcagtg ctttctcttg gactggcttg actcatggca ttctacacgt tattgctggt
     6721 ctaaatgtga ttttgccaag cttcttcagg acctataatt ttgcttgact tgtagccaaa
     6781 cacaagtaaa atgattaagc aacaaatgta tttgtgaagc ttggttttta ggttgttgtg
     6841 ttgtgtgtgc ttgtgctcta taataatact atccaggggc tggagaggtg gctcggagtt
     6901 caagagcaca gactgctctt ccagaagtcc tgagttcaat tcccagcaac cacatggtgg
     6961 ctcacaacca tctgtaatgg gatctgatgc cctcttctgg tgtgtctgaa gaccacaagt
     7021 gtattcacat taaataaata aatcctcctt cttcttcttt tttttttttt taaagagaat
     7081 actgtctcca gtagaattta ctgaagtaat gaaatacttt gtgtttgttc caatatggta
     7141 gccaataatc aaattactct ttaagcactg gaaatgttac caaggaacta atttttattt
     7201 gaagtgtaac tgtggacaga ggagccataa ctgcagactt gtgggataca gaagaccaat
     7261 gcagacttta atgtcttttc tcttacacta agcaataaag aaataaaaat tgaacttcta
     7321 gtatcctatt tgtttaaact gctagcttta cttaactttt gtgcttcatc tatacaaagc
     7381 tgaaagctaa gtctgcagcc attactaaac atgaaagcaa gtaatgataa ttttggattt
     7441 caaaaatgta gggccagagt ttagccagcc agtggtggtg cttgccttta tgcctttaat
     7501 cccagcactc tggaggcaga gacaggcaga tctctgagtt tgagcccagc ctggtctaca
     7561 catcaagttc tatctaggat agccaggaat acacacagaa accctgttgg ggaggggggc
     7621 tctgagattt cataaaatta taattgaagc attccctaat gagccactat ggatgtggct
     7681 aaatccgtct acctttctga tgagatttgg gtattatttt ttctgtctct gctgttggtt
     7741 gggtcttttg acactgtggg ctttctttaa agcctccttc ctgccatgtg gtctcttgtt
     7801 tgctactaac ttcccatggc ttaaatggca tggctttttg ccttctaagg gcagctgctg
     7861 agatttgcag cctgatttcc agggtggggt tgggaaatct ttcaaacact aaaattgtcc
     7921 tttaattttt tttttaaaaa atgggttata taataaacct cataaaatag ttatgaggag
     7981 tgaggtggac taatattaaa tgagtccctc ccctataaaa gagctattaa ggctttttgt
     8041 cttatactta actttttttt taaatgtggt atctttagaa ccaagggtct tagagtttta
     8101 gtatacagaa actgttgcat cgcttaatca gattttctag tttcaaatcc agagaatcca
     8161 aattcttcac agccaaagtc aaattaagaa tttctgactt ttaatgttaa tttgcttact
     8221 gtgaatataa aaatgatagc ttttcctgag gcagggtctc actatgtatc tctgcctgat
     8281 ctgcaacaag atatgtagac taaagttctg cctgcttttg tctcctgaat actaaggtta
     8341 aaatgtagta atacttttgg aacttgcagg tcagattctt ttatagggga cacactaagg
     8401 gagcttgggt gatagttggt aaaatgtgtt tcaagtgatg aaaacttgaa ttattatcac
     8461 cgcaacctac tttttaaaaa aaaaagccag gcctgttaga gcatgcttaa gggatcccta
     8521 ggacttgctg agcacacaag agtagttact tggcaggctc ctggtgagag catatttcaa
     8581 aaaacaaggc agacaaccaa gaaactacag ttaaggttac ctgtctttaa accatctgca
     8641 tatacacagg gatattaaaa tattccaaat aatatttcat tcaagttttc ccccatcaaa
     8701 ttgggacatg gatttctccg gtgaataggc agagttggaa actaaacaaa tgttggtttt
     8761 gtgatttgtg aaattgtttt caagtgatag ttaaagccca tgagatacag aacaaagctg
     8821 ctatttcgag gtctcttggt ttatactcag aagcacttct ttgggtttcc ctgcactatc
     8881 ctgatcatgt gctaggccta ccttaggctg attgttgttc aaataaactt aagtttcctg
     8941 tcaggtgatg tcatatgatt tcatatatca aggcaaaaca tgttatatat gttaaacatt
     9001 tgtacttaat gtgaaagtta ggtctttgtg ggtttgattt ttaattttca aaacctgagc
     9061 taaataagtc atttttacat gtcttacatt tggtggaatt gtataattgt ggtttgcagg
     9121 caagactctc tgacctagta accctaccta tagagcactt tgctgggtca caagtctagg
     9181 agtcaagcat ttcaccttga agttgagacg ttttgttagt gtatactagt ttatatgttg
     9241 gaggacatgt ttatccagaa gatattcagg actatttttg actgggctaa ggaattgatt
     9301 ctgattagca ctgttagtga gcattgagtg gcctttaggc ttgaattgga gtcacttgta
     9361 tatctcaaat aatgctggcc ttttttaaaa agcccttgtt ctttatcacc ctgttttcta
     9421 cataattttt gttcaaagaa atacttgttt ggatctcctt ttgacaacaa tagcatgttt
     9481 tcaagccata ttttttttcc tttttttttt tttttttggt ttttcgagac agggtttctc
     9541 tgtatagccc tggctgtcct ggaactcact ttgtagacca ggctggcctc gaactcagaa
     9601 atccgcctgc ctctgcctcc tgagtgccgg gattaaaggc gtgcaccacc acgcctggct
     9661 aagttggata ttttgttata taactataac caatactaac tccactgggt ggatttttaa
     9721 ttcagtcagt agtcttaagt ggtctttatt ggcccttcat taaaatctac tgttcactct
     9781 aacagaggct gttggtacta gtggcactta agcaacttcc tacggatata ctagcagatt
     9841 aagggtcagg gatagaaact agtctagcgt tttgtatacc taccagcttt atactacctt
     9901 gttctgatag aaatatttca ggacatctag agtgtactat aaggttgatg gtaagcttat
     9961 aaggaacttg aaagtggagt aactactcca tttctctgag gggagaatta aaatttttga
    10021 ccaagtgttg ttgagccact gagaatggtc tcagaacata acttcttaag gaaccttccc
    10081 agattgccct caacactgca ccacatttgg tcctgcttga acattgccat ggctcttaaa
    10141 gtcttaatta agaatattaa ttgtgtaatt attgtttttc ctcctttaga tcattccttg
    10201 aggacaggac agtgcttgtt taaggctata tttctgctgt ctgagcagca acaggtcttc
    10261 gagatcaaca tgatgttcat aatcccaaga tgttgccatt tatgttctca gaagcaagca
    10321 gaggcatgat ggtcagtgac ggcgcgtggc ctccgaggcc attaccctgt tatccctaca
    10381 taggcccaat tgccacggcc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg
    10441 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    10501 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    10561 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    10621 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    10681 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    10741 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    10801 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    10861 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    10921 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    10981 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    11041 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    11101 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    11161 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    11221 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    11281 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    11341 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    11401 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    11461 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    11521 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    11581 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    11641 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    11701 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    11761 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    11821 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    11881 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    11941 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    12001 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    12061 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    12121 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    12181 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    12241 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    12301 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    12361 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    12421 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    12481 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    12541 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    12601 gccac
//
