LOCUS       sequence-103277-        1085 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To construct C-terminal GFP fusions, single crossover integration 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Eberhardt A, Wu LJ, Errington J, Vollmer W, Veening JW
  TITLE     Cellular localization of choline-utilization proteins in 
            Streptococcus pneumoniae using novel fluorescent reporter systems.
  JOURNAL   Mol Microbiol. 2009 Oct;74(2):395-408. doi: 
            10.1111/j.1365-2958.2009.06872.x. Epub 2009 Sep 8.
  PUBMED    19737355
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2958.2009.06872.x"; journalName: "Mol Microbiol"; 
            date: "2009-10"; volume: "74"; issue: "2"; pages: "395-408"
COMMENT     Sequence Label: pAE03
FEATURES             Location/Qualifiers
     source          1..1085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1019..1040
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1055..1060,1061..1078,1079..1085)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 atcactcctt cttaattaca aatttttagc atctaattta acttcaattc ctattataca
       61 aaattttaag atactgcact atcaacacac tcttaagttt gcttctaagt cttatttcca
      121 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
      181 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
      241 catttcgttt ttctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
      301 ttcttattct tattttatca tcaacaatca caaatcactt gtgatttgtg ataagtgatt
      361 tgtgattaat atataaaagc cctctttaaa gggcttttat gtttattttg agaaagatat
      421 aaaatcaata tatccctttt ctccgatttt tacaacggca ttgtaggact ttctatcttt
      481 cgttttgatt ccttttacca gggtttcttt tccctctagt aattctttta catttgtttt
      541 ggtgagtttt ttctttctaa aatgttcagc taaagtaaac ttacattcag gataatttga
      601 acaaccataa aacgattttt ttaatacaat attgttgcca cacttaggac attttcctac
      661 aatacttttt tctgcttctt tttctttctg ttcctggtaa tcagaaaaat ttagtttttc
      721 tatatcgtta ggtacagctt ccagtaaatg aacaatgaat tttttgatat tcgtaataaa
      781 gttctcttga ttgccttctc ttttaccgat tttttttaaa tacgtttccc atttagccgt
      841 catttcagca ctcgttaaaa ggtgctgact ttcaactgcc tggcacaata attttccttt
      901 ttcagttaca acaagctcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      961 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     1021 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     1081 tgttg
//
