LOCUS       sequence-103284-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  yeast plasmid with Hsp82.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Jiang L, Mishra P, Hietpas RT, Zeldovich KB, Bolon DN
  TITLE     Latent effects of Hsp90 mutants revealed at reduced expression 
            levels.
  JOURNAL   PLoS Genet. 2013 Jun;9(6):e1003600. doi: 
            10.1371/journal.pgen.1003600. Epub 2013 Jun 27.
  PUBMED    23825969
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2013-06"; volume: "9(6):e1003600. doi"; pages: " 
            10.1371/journal.pgen.1003600. Epub 2013 Jun 27"
COMMENT     Sequence Label: p414CYC_Hsp82_CycTER
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..43
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             372..389
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
       61 catttggcga gcgttggttg gtggatcaag cccacgcgta ggcaatcctc gagcagatcc
      121 gccaggcgtg tatatatagc gtggatggcc aggcaacttt agtgctgaca catacaggca
      181 tatatatatg tgtgcgacga cacatgatca tatggcatgc atgtgctctg tatgtatata
      241 aaactcttgt tttcttcttt tctctaaata ttctttcctt atacattagg acctttgcag
      301 cataaattac tatacttcta tagacacgca aacacaaata cacacactaa tctagaacta
      361 gtatgggtgg tcaccaccat caccaccacg gtggtcatat ggctagtgaa acttttgaat
      421 ttcaagctga aattactcag ttgatgagtt tgatcatcaa caccgtctat tctaacaagg
      481 aaattttctt gagagaactg atatctaatg cctcggatgc gttggataaa attagataca
      541 aatctttgtc tgatccaaag caattggaaa cagaaccaga tctctttatt agaatcactc
      601 caaagccaga gcaaaaagtt ttggaaatca gagattctgg tattggtatg accaaggctg
      661 aattgattaa taacttgggt accattgcca agtctggtac caaagccttc atggaagctc
      721 tatctgctgg tgccgatgta tccatgattg gtcaattcgg tgttggtttt tactctttat
      781 tcttagttgc cgacagagtt caggttattt caaagagcaa cgacgacgaa caatacatct
      841 gggaatccaa cgctggtggt tctttcactg ttactctaga cgaagttaat gaaagaattg
      901 gtaggggtac catcttgagg ttattcttga aagatgacca attggagtac ttggaagaaa
      961 agagaataaa ggaagttatc aagagacatt ctgagttcgt ggcctaccca atccaattag
     1021 tcgtcaccaa ggaagttgaa aaggaagttc
//
