LOCUS       sequence-103369-        1236 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, N1 Cloning Vector, Excitation:505 / 569, 
            Emission:516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1236)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-MKS1-N-18
FEATURES             Location/Qualifiers
     source          1..1236
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      494..503
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             500..1183
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 atcttcatgg acttcggcca gagatttcgt tgaaggctac cggacttgag tctgctgtca
       61 ttcagatgcc acacctgact gtctccacat gagtccatga gctggtctcg ttgcagagct
      121 agagtctcat gcgctctctg agctgaggac ccatctatgt gaggatagca gaacctcaag
      181 gggggagcgc tgagccgctt tggatccgca ctgagacaac gggtactgtc accttccgat
      241 tgcactgcct gcagcagtcc cgggctttca tggaatcgaa ctcctccaga aacagatgcg
      301 gagtgtgttg gaccgtctgg aagggttcag ccagcagagt tccacgcaca acgtgctaga
      361 agcattccgt cgagcccggc gtcggatgca ggaggcccga gagagcctcc cccaggacct
      421 tgtgagcccc acgggaacgt tgaccgggtc gagaggttcc agcggggtac cgcgggcccg
      481 ggatccaccg gtcgccacca tggtgagtgc gattaagcca gacatgagga tcaaactccg
      541 tatggaaggc aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc
      601 ttatgaggga aaacagacca tggatcttga agtcaaagag ggcggacctc tgccttttgc
      661 ctttgatatc ctgaccactg cattccatta cggcaacagg gtattcgtga aatatccaga
      721 caacatacaa gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt
      781 gactttcgaa gacgggggca tttgcaatgc cagaaacgac ataacaatgg aaggggacac
      841 tttctataat aaagttcgat tttatggtac caactttccc gccaatggtc cagttatgca
      901 gaagaagacg ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct
      961 gacgggtgat attgagatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt
     1021 cagaactact tacaaagcta aggagaaggg tgtcaagtta ccaggcgccc actttgtgga
     1081 ccacgccatt gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca
     1141 tgctgttgct cattctggat tgcctgacaa tgccagacga taagcggccg cgactctaga
     1201 tcataatcag ccataccaca ttgtagaagg aagcct
//
