LOCUS       sequence-10340-e        9578 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Meylan E, Dooley AL, Feldser DM, Shen L, Turk E, Ouyang C, Jacks T
  TITLE     Requirement for NF-kappaB signalling in a mouse model of lung 
            adenocarcinoma.
  JOURNAL   Nature. 2009 Nov 5;462(7269):104-7. doi: 10.1038/nature08462. Epub 
            2009 Oct 21.
  PUBMED    19847165
REFERENCE   2  (bases 1 to 9578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature08462"; journalName: "Nature"; date: "2009-11-5- 5"; 
            volume: "462"; issue: "7269"; pages: "104-7"
COMMENT     Sequence Label: Ubc.IkBSR-Flag Pgk.Cre
FEATURES             Location/Qualifiers
     source          1..9578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1686..1919
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2104..2148
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2446..2563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2632..3841
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3678..3697
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4816..4839
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        4859..5358
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4894..4917)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5291..5310
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5362..5382
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5383..6411
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    6467..7055
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6520..6540)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6938..6949)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7058..7074)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7059..7075)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7584..7764
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7826..8414)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7906..7925)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8585..9376,9377..9445))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9208..9227
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9446..9550)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
     1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2581 cagcagagat ccagtttggt tagtaccggg cccgctctag acggttgatc tggcctccgc
     2641 gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag
     2701 acgaagggcg caggagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     2761 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     2821 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     2881 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     2941 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     3001 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     3061 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     3121 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     3181 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     3241 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     3301 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     3361 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatgcggtgc
     3421 cgttgggcag tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc
     3481 cgttctgttg gcttataatg cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt
     3541 tctccgtcgc aggacgcagg gttcgggcct agggtaggct ctcctgaatc gacaggcgcc
     3601 ggacctctgg tgaggggagg gataagtgag gcgtcagttt ctttggtcgg ttttatgtac
     3661 ctatcttctt aagtagctga agctccggtt ttgaactatg cgctcggggt tggcgagtgt
     3721 gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt
     3781 tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga
     3841 cgaagctaac gcgctagcaa agaattcgcc accatgtttc agccagctgg gcacggccag
     3901 gactgggcca tggagggccc gcgggatggc ctcaagaagg agcgcttggt ggacgatcgc
     3961 cacgacgccg gcctggacgc catgaaggac gaggagtacg agcaaatggt gaaggagctg
     4021 cgggagatcc gcctgcagcc gcaggaggcg ccgctggccg ccgagccctg gaagcagcag
     4081 ctcacggagg acggagactc gttcctgcac ttggcaatca tccacgaaga gaagccgctg
     4141 accatggaag tcattggtca ggtgaaggga gacctggcct tcctcaactt ccagaacaac
     4201 ctgcagcaga ctccactcca cttggctgtg atcaccaacc agccaggaat tgctgaggca
     4261 cttctgaaag ctggctgtga tcctgagctc cgagactttc gaggaaatac ccctctacat
     4321 cttgcctgtg agcagggctg cctggccagt gtagcagtct tgacgcagac ctgcacaccc
     4381 cagcatctcc actccgtcct gcaggccacc aactacaatg gccacacgtg tctgcaccta
     4441 gcctctatcc acggctacct ggccatcgtg gagcacttgg tgactttggg tgctgatgtc
     4501 aacgctcagg agccctgcaa tggccggaca gccctccacc ttgcggtgga cctgcagaat
     4561 cctgacctgg tttcgctctt gttgaaatgt ggggctgatg tcaacagggt aacctaccaa
     4621 ggctactccc cctaccagct tacctggggc cgcccaagta cccggataca gcagcagctg
     4681 ggccagctga ccctggaaaa tctccagatg ctacccgaga gcgaggatga ggagagctat
     4741 gacacggagt cagaattaac agaggatgag ctgccctatg atgactgtgt gtttggaggc
     4801 cagcgtctga cattagatta caaagacgat gacgataaat aagcggccgc attctaccgg
     4861 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     4921 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     4981 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     5041 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     5101 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     5161 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     5221 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     5281 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     5341 tcatctccgg gacccgggat gcccaagaag aagaggaagg tgtccaattt actgaccgta
     5401 caccaaaatt tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg
     5461 atggacatgt tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc
     5521 gtttgccggt cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa
     5581 cctgaagatg ttcgcgatta tcttctatat cttcaggcgc gcggtctggc agtaaaaact
     5641 atccagcaac atttgggcca gctaaacatg cttcatcgtc ggtccgggct gccacgacca
     5701 agtgacagca atgctgtttc actggttatg cggcggatcc gaaaagaaaa cgttgatgcc
     5761 ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg atttcgacca ggttcgttca
     5821 ctcatggaaa atagcgatcg ctgccaggat atacgtaatc tggcatttct ggggattgct
     5881 tataacaccc tgttacgtat agccgaaatt gccaggatca gggttaaaga tatctcacgt
     5941 actgacggtg ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgca
     6001 ggtgtagaga aggcacttag cctgggggta actaaactgg tcgagcgatg gatttccgtc
     6061 tctggtgtag ctgatgatcc gaataactac ctgttttgcc gggtcagaaa aaatggtgtt
     6121 gccgcgccat ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca
     6181 actcatcgat tgatttacgg cgctaaggat gactctggtc agagatacct ggcctggtct
     6241 ggacacagtg cccgtgtcgg agccgcgcga gatatggccc gcgctggagt ttcaataccg
     6301 gagatcatgc aagctggtgg ctggaccaat gtaaatattg tcatgaacta tatccgtaac
     6361 ctggatagtg aaacaggggc aatggtgcgc ctgctggaag atggcgatta gccatgttta
     6421 agggttccgg ttccactagg tacaattcga tatcaagctt atcgataatc aacctctgga
     6481 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     6541 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     6601 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6661 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6721 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6781 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6841 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     6901 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     6961 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7021 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgatcgag
     7081 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     7141 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     7201 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     7261 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     7321 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     7381 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     7441 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     7501 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     7561 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7621 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7681 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7741 agtcagtgtg gaaaatctct agcagcatgt gagcaaaagg ccagcaaaag gccaggaacc
     7801 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7861 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7921 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7981 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8041 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8101 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8161 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8221 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     8281 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8341 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8401 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8461 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8521 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8581 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8641 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8701 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8761 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8821 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8881 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8941 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9001 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9061 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9121 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9181 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9241 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9301 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9361 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9421 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9481 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9541 gggttccgcg cacatttccc cgaaaagtgc cacctgac
//
