LOCUS       sequence-103420-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Fujii H, Fujita T
  TITLE     An enChIP system for the analysis of genome functions in budding 
            yeast.
  JOURNAL   Biol Methods Protoc. 2022 Oct 17;7(1):bpac025. doi: 
            10.1093/biomethods/bpac025. eCollection 2022.
  PUBMED    36325175
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Methods Protoc."; date: "2022-10-17"; volume: "7(1):bpac025. doi"; 
            pages: " 10.1093/biomethods/bpac025. eCollection 2022"
COMMENT     Sequence Label: 3xFLAG-dCas9/pTEF1p-CYC1t
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..37)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actcgagaag agatcacacc ttcctcttct tcttggggtc agccctgctg tctccaccga
       61 gctgagagag gtcgattctt gtttcataga gccccgtaat tgactgatga atcagtgtgg
      121 cgtccaggac ctcctttgta gaggtgtacc gctttctgtc tatggtggtg tcgaagtact
      181 tgaaggctgc aggcgcgccc aagttggtca gagtaaacaa gtggataatg ttttctgcct
      241 gctccctgat gggcttatcc ctgtgcttat tgtaagcaga aagcacctta tcgaggttag
      301 cgtcggcgag gatcactctt ttggagaatt cgcttatttg ctcgatgatc tcatcaaggt
      361 agtgtttgtg ttgttccacg aacagctgct tctgctcatt atcttcggga gaccctttga
      421 gcttttcata gtggctggcc agatacaaga aattaacgta tttagagggc agtgccagct
      481 cgttaccttt ctgcagctcg cccgcactag cgagcattcg tttccggccg ttttcaagct
      541 caaagagaga gtacttggga agcttaatga tgaggtcttt tttgacctct ttatatcctt
      601 tcgcctcgag aaagtcgatg gggttttttt cgaagcttga tcgctccatg attgtgatgc
      661 ccagcagttc cttgacgctt ttgagttttt tagacttccc tttctccact ttggccacaa
      721 ccagtacact gtaagcgact gtaggagaat cgaatccgcc gtatttcttg gggtcccaat
      781 cttttttgcg tgcgatcagc ttgtcgctgt tccttttcgg gaggatactt tccttggaga
      841 agcctccggt ctgtacttcg gtctttttaa cgatgttcac ctgcggcatg gacaggacct
      901 tccggactgt cgcgaaatcc ctacccttgt cccacacgat ttctcctgtt tctccgtttg
      961 tttcgataag tggtcgcttc cgaatctctc cattggccag tgtaatctcg gtcttgaaaa
//
