LOCUS       sequence-103457-         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Destination vector for use 
            with pMuLE Entry vectors. Co-expresses iRFP under the control of a 
            PGK promoter. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE Lenti Dest iRFP
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgacctgcag cccaagctta ccacactcct gcatctgccg ccaccatggc ggaaggatcc
       61 gtcgccaggc agcctgacct cttgacctgc gacgatgagc cgatccatat ccccggtgcc
      121 atccaaccgc atggactgct gctcgccctc gccgccgaca tgacgatcgt tgccggcagc
      181 gacaaccttc ccgaactcac cggactggcg atcggcgccc tgatcggccg ctctgcggcc
      241 gatgtcttcg actcggagac gcacaaccgt ctgacgatcg ccttggccga gcccggggcg
      301 gccgtcggag caccgatcac tgtcggcttc acgatgcgaa aggacgcagg cttcatcggc
      361 tcctggcatc gccatgatca gctcatcttc ctcgagctcg agcctcccca gcgggacgtc
      421 gccgagccgc aggcgttctt ccgccgcacc aacagcgcca tccgccgcct gcaggccgcc
      481 gaaaccttgg aaagcgcctg cgccgccgcg gcgcaagagg tgcggaagat taccggcttc
      541 gatcgggtga tgatctatcg cttcgcctcc gacttcagcg gcgaagtgat cgcagaggat
      601 cggtgcgccg aggtcgagtc aaaactaggc ctgcactatc ctgcctcaac cgtgccggcg
      661 caggcccgtc ggctctatac catcaacccg gtacggatca ttcccgatat caattatcgg
      721 ccggtgccgg tca
//
