LOCUS       sequence-10347-e        4700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Pastrana DV, Tolstov YL, Becker JC, Moore PS, Chang Y, Buck CB
  TITLE     Quantitation of human seroresponsiveness to Merkel cell 
            polyomavirus.
  JOURNAL   PLoS Pathog. 2009 Sep . 5(9):e1000578.
  PUBMED    19750217
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog. 2009 Sep . 5(9):e1000578."
COMMENT     Sequence Label: ph2m
FEATURES             Location/Qualifiers
     source          1..4700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(8..28)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13..342
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      193..328
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      367..376
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(373..375,376..378,379..1092)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(373..375,376..378,379..1089)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(418..439)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(679..698)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1026..1047
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1117..1238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1128..1147)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1182..1201
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1439..1650
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1663..1931
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     protein_bind    1973..1997
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2745..2769)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    2789..3361
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     rep_origin      3532..4120
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4021..4040
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        4206..4253
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        4245..4263
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cccctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
       61 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
      121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
      181 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
      241 tgactaattt tttttattta tacagaggcc gaggccgcct cggcctctga gctattccag
      301 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttga ttgggatcca
      361 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      421 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      481 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      541 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      601 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      661 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      721 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      781 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
      841 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
      901 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      961 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1021 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1081 ctgtacaagt aaagcggccg cttcgagcag acatgataag atacattgat gagtttggac
     1141 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     1201 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     1261 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     1321 aatgtggtaa aatcgataag gatccgggct ggcgtaatag cgaagaggcc cgcaccgatc
     1381 gcccttccca acagttgcgg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg
     1441 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     1501 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     1561 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     1621 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     1681 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     1741 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     1801 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     1861 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     1921 cgttacagat ccaagctgtg accggcgcct acgtaagtga tagcttgatc aaacaagttt
     1981 gtacaaaaaa gcaggctgcg gccgcaacaa atgggcggga tcattaccct gctcgcaaac
     2041 atcggcgaga tcgccaccga gctgtccgca acaaccggcg tgacactgga agccatcttg
     2101 accggcgagg ccctggccgc actcgaggcc gagattagta gcctgatgac catcgaaggc
     2161 atcagcggga tcgaagccct ggcacagctg ggctttaccg ccgagcaatt cagcaacttc
     2221 agcctggtcg ccagcctcgt caatcagggc ctgacctacg ggttcatctt gcaaaccgtg
     2281 tccggcatcg ggagcctgat caccgtcggc gtccgcctga gcagggaaca ggtcagcctg
     2341 gtcaaccgcg acgtcagctg ggtcggctcc aacgaagtcc tgcgccacgc cttgatggca
     2401 ttctcactgg acccgctcca atgggagaac agcctgctgc acagcgtcgg ccaggacatc
     2461 ttcaacagcc tgagccccac aagccgcctg caaatccaga gcaacttggt caacctcatc
     2521 ctgaactcaa gatgggtgtt ccaaaccacc gccagccaga accaggggct gctgagcggc
     2581 gaagccatcc tgatccccga acacatcggc ggcaccctgc aacagcagac acccgactgg
     2641 ctgctgccac tggtgctggg gttgtccggc tacatcagcc ccgagctgca agtcatcgag
     2701 gacgggacaa agaagaagtc aatcattcat ctctgagtgc tagcacccag ctttcttgta
     2761 caaagtggtt cgatctagaa tggctagcat tatccctaat acctgccacc ccactcttaa
     2821 tcagtggtgg aagaacggtc tcagaactgt ttgtttcaat tggccattta agtttagtag
     2881 taaaagactg gttaatgata acaatgcatc gtaaaacctt cagaaggaaa ggagaatgtt
     2941 ttgtggacca ctttggtttt cttttttgcg tgtggcagtt ttaagttatt agtttttaaa
     3001 atcagtactt tttaatggaa acaacttgac caaaaatttg tcacagaatt ttgagaccca
     3061 ttaaaaaagt taaatgagaa acctgtgtgt tcctttggtc aacaccgaga catttaggtg
     3121 aaagacatct aattctggtt ttacgaatct ggaaacttct tgaaaatgta attcttgagt
     3181 taacacttct gggtggagaa tagggttgtt ttccccccac ataattggaa ggggaaggaa
     3241 tatcatttaa agctatggga gggtttcttt gattacaaca ctggagagaa atgcagcatg
     3301 ttgctgattg cctgtcacta aaacaggcca aaaactgagt ccttgggttg catagaaagc
     3361 ttcatgttgc taaaccaatg ttaagtgaat ctttggaaac aaaatgtttc caaattactg
     3421 ggatgtgcat gttgaaacgt gggttaatta actagccatg accaaaatcc cttaacgtga
     3481 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3541 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3601 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3661 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3721 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3781 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3841 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3901 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3961 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4021 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4081 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4141 tttacggttc ctggcctttt gctggccttt tgctcacatg ttcttaatta aatttttcaa
     4201 aagtagttga caattaatca tcggcatagt atatcggcat agtataatac gactcactat
     4261 aggagggcca tcatggccaa gttgaccagt gctgtcccag tgctcacagc cagggatgtg
     4321 gctggagctg ttgagttctg gactgacagg ttggggttct ccagagattt tgtggaggat
     4381 gactttgcag gtgtggtcag agatgatgtc accctgttca tctcagcagt ccaggaccag
     4441 gtggtgcctg acaacaccct ggcttgggtg tgggtgagag gactggatga gctgtatgct
     4501 gagtggagtg aggtggtctc caccaacttc agggatgcca gtggccctgc catgacagag
     4561 attggagagc agccctgggg gagagagttt gccctgagag acccagcagg caactgtgtg
     4621 cactttgtgg cagaggagca ggactgagga taagaattgt aacaaaaaac cccgccccgg
     4681 cggggttttt tgttaattaa
//
