LOCUS       sequence-103500-        1107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of cyclin B1 non-degradable L45A mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Mansfeld J, Collin P, Collins MO, Choudhary JS, Pines J
  TITLE     APC15 drives the turnover of MCC-CDC20 to make the spindle assembly 
            checkpoint responsive to kinetochore attachment.
  JOURNAL   Nat Cell Biol. 2011 Sep 18;13(10):1234-43. doi: 10.1038/ncb2347.
  PUBMED    21926987
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2011-09-18- 18"; volume: "13"; issue: "10"; 
            pages: "1234-43"
COMMENT     Sequence Label: pcDNA5 FRT/TO/STRP 3x FLAG cyclin B1 L45A (1391)
FEATURES             Location/Qualifiers
     source          1..1107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..95)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(108..131)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(168..191)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    202..226
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgggccctct agactcgagc ggccgcctac ttgtcatcgt catccttgta gtcgatgtca
       61 tgatctttat aatcaccgtc atggtctttg tagtcagccc gggatccttt ttcgaactgc
      121 gggtggctcc acgatccacc tcccgatcca cctccggaac ctccaccttt ctcgaactgc
      181 gggtggctcc acgctgagat caccactttg tacaagaaag ctgggtacac ctttgccaca
      241 gccttggcta aatcttgaac tagtgcagaa ttcagctgtg gtagagtgct gatcttagca
      301 tgcttcgatg tggcatactt gttcttgaca gtcatgtgct ttgtaagtcc ttgatttacc
      361 atgactacat tcttagccag gtgctgcata actggaagaa gagattcttc agtatatgac
      421 aggtaatgtt gtagagttgg tgtccattca ccattatcca gaattttcag tgctaagcaa
      481 aaagctcctg ctgcaatttg agaaggagga aagtgcacca tgtcatagtc caacatagtt
      541 agttccatca ggtatttggc caaagtatgt tgctcgacat caacctctcc aatcttagat
      601 gctctccgaa ggaagtgcaa aggtagaggc cgacccagac caaagtttaa agctcttaga
      661 atcttcattt ccatctgtct gatttggtgc ttagtataag tgttgtcagt cacaaaagca
      721 aagtcaccaa tttctggagg gtacatttct tcatatttgc ttgcaataaa catggcagtg
      781 acaccaacca gctgcagcat cttcttgggc acacaattat tctgcatgaa ccgatcaata
      841 atggagacag tcatgtacat ggtctcctgc aacaacctga atttcatttg aacctgtact
      901 agccagtcaa ttaggatggc tctcatgttt ccagtgactt cccgacccag taggtatttt
      961 ggtctgactg cttgctcttc ctcaagttgt ctcagataag cataaatatc tttcacatat
     1021 tcactacaaa ggtttggatc agctccatct tctgcatcca catcatttac tgcaagaatt
     1081 acatcagaga aagcctgaca caggtct
//
