LOCUS       sequence-103522-         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV
            promoter and tdTomato module. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SFFV tdTomato L5-L2
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..113
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     126..142
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     127..143
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(250..273)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(448..467)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgcagctgga tggcaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
       61 aattgataag caatgctttc ttataatgcc aactttgtac aagaaagctg ggtacctcta
      121 gaggctcgag gtcgacggta tcgataagct tgatatcgaa ttcttacttg tacagctcgt
      181 ccatgccgta caggaacagg tggtggcggc cctcggagcg ctcgtactgt tccacgatgg
      241 tgtagtcctc gttgtgggag gtgatgtcca gcttggtgtc cacgtagtag tagccgggca
      301 gttgcacggg cttcttggcc atgtagatgg tcttgaactc caccaggtag cggccgccgt
      361 ccttcagctt cagggcctgg tggatctcgc ccttcagcac gccgtcgcgg gggtacaggc
      421 gctcggtgga ggcctcccag cccatggtct tcttctgcat tacggggccg tcggggggga
      481 agttggtgcc gcgcatcttc accttgtaga tcagcgtgcc gtcctgcagg gaggagtcct
      541 gggtcacggt caccagaccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct
      601 cggggaagga cagcttcttg taatcgggga tgtcggcggg gtgcttcacg tacgccttgg
      661 agccgtacat gaact
//
