LOCUS       sequence-103565-         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 
            promoter and human codon optimized Cas9 module. Compatible with 
            MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SV40-hCas9 L3-L2
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(118..138)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tacaagaaag ctgggtatct agagtcgcgg ccgctcacac
      121 cttcctcttc ttcttggggt cagccctgct gtctccaccg agctgagaga ggtcgattct
      181 tgtttcatag agccccgtaa ttgactgatg aatcagtgtg gcgtccagga cctcctttgt
      241 agaggtgtac cgctttctgt ctatggtggt gtcgaagtac ttgaaggctg caggcgcgcc
      301 caagttggtc agagtaaaca agtggataat gttttctgcc tgctccctga tgggcttatc
      361 cctgtgctta ttgtaagcag aaagcacctt atcgaggtta gcgtcggcga ggatcactct
      421 tttggagaat tcgcttattt gctcgatgat ctcatcaagg tagtgtttgt gttgttccac
      481 gaacagctgc ttctgctcat tatcttcggg agaccctttg agcttttcat agtggctggc
      541 cagatacaag aaattaacgt atttagaggg cagtgccagc tcgttacctt tctgcagctc
      601 gcccgcacta gcgagcattc gtttccggcc gttttcaagc tcaaagagag agtacttggg
      661 aagcttaatg atgaggtc
//
