LOCUS       sequence-103585-         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 
            promoter for shRNA expression. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR U6 L1-R5
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(39..162)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(397..637)
                     /label=U6 promoter
                     /note="Truncated RNA polymerase III promoter for human U6 
                     snRNA used in pSIREN vectors such as 
                     pSIREN-RetroQ-ZsGreen1. Expected transcription start site 
                     is 8bp downstream of this feature."
     primer_bind     complement(447..466)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(617..637)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tgcagtacag gtcactaata ccatctaagt agttgattca tagtgactgg atatgttgtg
       61 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
      121 tatcatttta cgtttctcgt tcaacttttg tatacaaagt tgtcgaattc aaaaaagagc
      181 tcggactcgg gcgccggcct ggagctgggc atcgcgtcct ccccgacgcc tggctccacc
      241 gcgtcgacgg gcggcaaggc ggacgacccc agctggtgca agacgcccag tggccacatc
      301 aagcggccca tgaacgcctt tatggtgtgg tcgcagatcg agcggcgcaa gatcatggag
      361 cagtcgcccg acatgcacaa cgccgagatc tgtttcgtcc tttccacaag atatataaag
      421 ccaagaaatc gaaatacttt caagttacgg taagcatatg atagtccatt ttaaaacata
      481 attttaaaac tgcaaactac ccaagaaatt attactttct acgtcacgta ttttgtacta
      541 atatctttgt gtttacagtc aaattaattc caattatctc tctaacagcc ttgtatcgta
      601 tatgcaaata tgaaggaatc atgggaaata ggccctcggt gaagggggcg gccgctcgag
      661 gctagtctcg gatcgataga gcctgc
//
