LOCUS       sequence-103636-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector; TetON promoter driving mouse Ngn2 
            and Ngn1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Busskamp V, Lewis NE, Guye P, Ng AH, Shipman SL, Byrne SM, Sanjana 
            NE, Murn J, Li Y, Li S, Stadler M, Weiss R, Church GM
  TITLE     Rapid neurogenesis through transcriptional activation in human stem 
            cells.
  JOURNAL   Mol Syst Biol. 2014 Nov 17;10(11):760. doi: 10.15252/msb.20145508.
  PUBMED    25403753
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-17"; volume: "10(11):760. doi"; pages: " 
            10.15252/msb.20145508"
COMMENT     Sequence Label: pLV_TRET_Ngn2‐2A‐Ngn1
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    50..68
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    85..103
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    121..139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    157..175
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    192..210
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    228..246
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        258..296
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     293..317
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    336..360
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agttggctcc gaattcgccc ttcaggtccg aggttctaga cgagtttact ccctatcagt
       61 gatagagaac gatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
      121 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
      181 tgtcgagttt atccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
      241 tagagaacgt atgtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt
      301 tagtgaaccg tcagatcgca aagggcgaat tcgacccaag tttgtacaaa aaagcaggct
      361 gagccaccat gttcgtcaaa tctgagactc tggagttgaa ggaggaagag gaggtactga
      421 tgctgctggg ctcggcttcc ccggcctcgg cgaccctgac cccgatgtcc tccagcgcgg
      481 acgaggagga ggacgaggag ctgcgccggc cgggctccgc gcgtgggcag cgtggagcgg
      541 aagccgggca gggggtgcag ggcagtccgg cgtcgggtgc cgggggttgc cggccagggc
      601 ggctgctggg cctgatgcac gagtgcaagc gtcgcccgtc gcgctcacgg gccgtctccc
      661 gaggtgccaa gacggcggag acggtgcagc gcatcaagaa gacccgcagg ctcaaggcca
      721 acaaccgcga gcgcaaccgc atgcacaacc taaacgccgc gctggacgcg ctgcgcgagg
      781 tgctgcccac cttccccgag gatgccaagc tcacgaagat cgagacgctg cgcttcgccc
      841 acaattacat ctgggcgctc accgagactc tgcgcctggc
//
