LOCUS       sequence-103637-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human Rab5B fluorescently tagged with GFP on the N-terminus for 
            mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Rowland AA, Chitwood PJ, Phillips MJ, Voeltz GK
  TITLE     ER Contact Sites Define the Position and Timing of Endosome Fission.
  JOURNAL   Cell. 2014 Nov 20;159(5):1027-41. doi: 10.1016/j.cell.2014.10.023.
  PUBMED    25416943
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.10"; journalName: "Cell"; date: "2014-11-20- 
            20"; volume: "159"; issue: "5"; pages: "1027-41"
COMMENT     Sequence Label: GFP-Rab5B
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      43..52
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             49..765
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
ORIGIN
        1 agctggttta gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag
       61 ggcgccgagc tgttcaccgg catcgtgccc atcctgatcg agctgaatgg cgatgtgaat
      121 ggccacaagt tcagcgtgag cggcgagggc gagggcgatg ccacctacgg caagctgacc
      181 ctgaagttca tctgcaccac cggcaagctg cctgtgccct ggcccaccct ggtgaccacc
      241 ctgagctacg gcgtgcagtg cttctcacgc taccccgatc acatgaagca gcacgacttc
      301 ttcaagagcg ccatgcctga gggctacatc caggagcgca ccatcttctt cgaggatgac
      361 ggcaactaca agtcgcgcgc cgaggtgaag ttcgagggcg ataccctggt gaatcgcatc
      421 gagctgaccg gcaccgattt caaggaggat ggcaacatcc tgggcaataa gatggagtac
      481 aactacaacg cccacaatgt gtacatcatg accgacaagg ccaagaatgg catcaaggtg
      541 aacttcaaga tccgccacaa catcgaggat ggcagcgtgc agctggccga ccactaccag
      601 cagaataccc ccatcggcga tggccctgtg ctgctgcccg ataaccacta cctgtccacc
      661 cagagcgccc tgtccaagga ccccaacgag aagcgcgatc acatgatcta cttcggcttc
      721 gtgaccgccg ccgccatcac ccacggcatg gatgagctgt acaagtccgg actcagatct
      781 cgagctatga ctagcagaag cacagctagg cccaatgggc aacccc
//
