LOCUS       sequence-103639-         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40
            promoter and firefly luciferase module. Compatible with MultiSite 
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SV40 Luc2 (FF-Luciferase) L3-L2
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tacaagaaag ctgggtatct agaattatta cacggcgatc
      121 ttgccgccct tcttggcctt aatgagaatc tcgcggatct tgcgggcgtc caacttgccg
      181 gtcagtcctt taggcacctc gtccacgaac acaacaccac cgcgcagctt cttggcggtt
      241 gtaacctggc tggccacata gtccacgatc tccttctcgg tcatggtttt accgtgttcc
      301 agcacgacga ctgcggcggg cagctcgccg gcatcgtcgt cgggcaggcc ggcgaccccg
      361 gcgtcgaaga tgttggggtg ttgcagcagg atgctctcca gttcggctgg ggctacctgg
      421 tagcccttgt atttgatcag gctcttcagc cggtccacga tgaagaagtg ctcgtcctcg
      481 tcccagtagg cgatgtcgcc gctgtgcagc cagccgtcct tgtcgatgag agcgtttgta
      541 gcctcggggt tgttaacgta gccgctcatg atcatggggc cacggacgca cagctcgccg
      601 cgctggttca cacccagtgt cttaccggtg tccaagtcca ccaccttagc ctcg
//
