LOCUS       sequence-103806-         405 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Part of Multibow set of constructs--each FP gene is initially not 
            expressed and then adopts a permanent ON or OFF status upon 
            Cre-mediated recombination. Encodes nuclear dTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Xiong F, Obholzer ND, Noche RR, Megason SG
  TITLE     Multibow: digital spectral barcodes for cell tracing.
  JOURNAL   PLoS One. 2015 May 26;10(5):e0127822. doi: 
            10.1371/journal.pone.0127822. eCollection 2015.
  PUBMED    26010570
REFERENCE   2  (bases 1 to 405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-26"; volume: "10(5):e0127822. doi"; pages: " 
            10.1371/journal.pone.0127822. eCollection 2015"
COMMENT     Sequence Label: pMTB-Multibow-hR
FEATURES             Location/Qualifiers
     source          1..405
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        290..308
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     290..307
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(363..396)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
ORIGIN
        1 tttttactga gccctacgac tctggctgag tgccacaccg ccggcagccg caaagcggct
       61 caaaccattg ccttttatgg taataatgag agaatgcaga gggacttcct ttgtctggca
      121 tatctgaggc gcgcgctgtc actagcgccc accagcggtc agactgtaga atgcagagca
      181 aaacaggaag ttgactccag atggtcacat gcttgctgaa acgcctctcc ctggcagcag
      241 tgcaattcta acagtttccc tttcttttac agttcgccat cctaggccta tttaggtgac
      301 actatagaag agtacaagct acttgttctt tttgcaggat ccgccaccat gactagctcg
      361 agataacttc gtatagtata ccttatacga agttatcgag ctcat
//
