LOCUS       sequence-103811-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NpuDnaB(delta283)dN11 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSABAD14-111
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..22)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             618..640
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(725..744)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 cttaatgatg atgatgatga tggaattccg ttacggtgta ggttttggta gcgtcgtcgt
       61 aggtccattc accgtcaaca ccgttgtcgt tagcgtactg tttgaaaact ttttccgcgg
      121 tagcagcgtc aacagcttcg gtggtggttt cacctttcag ggttttaccg ttcaggataa
      181 gtttgtactg ggtaccaatg gaattgtgaa caataatatt atttgcaaca aaattatgca
      241 aaccaggaac ggtgaggtca aacacttctt cctcaccact atattcaatt gaaactattt
      301 catcccaata aatatcactt ttggcaagag taccagaact ggggagattt cttggtaaag
      361 cgagatgttc tttaggagtt aattcatcaa gtcttttcca gccattaatt gttaaaaact
      421 tgtgattacc tgtggcacgg attttccgcc ccaatcgagt tgtcaaagta aacagaggct
      481 ttataccagt cgaaaaagca ttgctaacaa ttgccttttc tagctgcatt gtagcttcgt
      541 tcaatgccca aactgcaaaa ccagatttac ctactaattc cttaattggc acttgcagtc
      601 cggatcccat atgtatatct ccttcttaaa gttaaacaaa attatttcta gcccaaaaaa
      661 acgggtatgg agaaacagta gagagttgcg ataaaaagcg tcaggtagga tccgctaatc
      721 ttatggataa aaatgctatg gcatagcaaa gtgtgacgcc gtgcaaataa tcaatgtgga
      781 cttttctgcc gtgattatag acacttttgt tacgcg
//
