LOCUS       sequence-103853-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NpuDnaEN35 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-218
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..60)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(197..487)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             complement(494..511)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    567..591
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(591..610)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(592..610)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        656..733
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(753..772)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 agctgcgcta gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt
       61 accagactcg agtgcggccg caagcttaat tatcaacgct ataaacagta cattcgatgc
      121 gcttttctac aattttacca atcggtaata atccatattc tactgtcaat atttccgttt
      181 catagcttaa acaggatcca ccaatctgtt ctctgtgagt ctcaataata tcgttatcct
      241 ccatgtccaa atcttcaggg gtctgatcag cttgaattct aataccgtcg tacaagaatc
      301 ttaaggagtc catttcctta ccctgtcttt tagcgaacgc ttccatcagc cttcttaaag
      361 gagtggtctt tttgatctta aagaagattt ctgaagatcc atcggacacc tttaaattga
      421 tgtgagtctc aggcttgact tctggcttga cctctggctt agcttcttga ttgacttctg
      481 agtcactagt gctgtgatga tgatgatgat ggctgctgcc catggtatat ctccttatta
      541 aagttaaaca aaattatttc tacaggggaa ttgttatccg ctcacaattc ccctatagtg
      601 agtcgtatta atttcctaat gcaggagtcg cataagggag agcgtcgaga tcccggacac
      661 catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
      721 cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
      781 ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgc
//
