LOCUS       sequence-10392-e       18033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18033)
  AUTHORS   D'Agostino I, Merritt C, Chen PL, Seydoux G, Subramaniam K
  TITLE     Translational repression restricts expression of the C. elegans 
            Nanos homolog NOS-2 to the embryonic germline.
  JOURNAL   Dev Biol. 2006 Apr 1. 292(1):244-52.
  PUBMED    16499902
REFERENCE   2  (bases 1 to 18033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2006 Apr 1. 292(1):244-52."
COMMENT     Sequence Label: pID3.01B
FEATURES             Location/Qualifiers
     source          1..18033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     624..643
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     668..684
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2641..2652)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(9956..9972)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10026..10054)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     10802..10822
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    10917..11041
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(11066..11071,11072..11089,11090..11096)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             11150..11809
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(11216..11235)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             12151..12456
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     12335..12354
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(12497..12621)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(15842..15860)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(15881..15897)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15881..15897)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15894..15916)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15905..15921
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15929..15935,15936..15953,15954..15959))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(16112..16129)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16283..16871)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(17042..17833,17834..17902))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17665..17684
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17903..18007)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccgggc
      661 cccccctcga ggtcgacggt atcgataagc ttcagtaaaa gaagtagaat tttatagttt
      721 tttttctgtt tgaaaaattc tccccatcaa tgttctttca aataaataca tcactaatgc
      781 aaagtattct ataacctcat atctaaattc ttcaaaatct taacatatct tatcattgct
      841 ttaagtcaaa cgtaacatta aaaaaaatgt tttggaaaat gtgtcaagtc tctcaaaatt
      901 cagtttttta aaccactcct atagtcctat agtcctatag ttacccatga aatccttata
      961 tattactgta aaatgtttca aaaaccattg gcaaattgcc agaactgaaa atttccggca
     1021 aattggggaa ccggcaaatt gccaatttgc tgaatttgcc ggaaaaacgg taattgccga
     1081 aagtttttga cacgaaaatg gcaaattgtg gttttaaaat tttttttttt ggaaatttca
     1141 gaatttcaat tttaatcggc aaaactgtag gcatcctaag aatgttccta catctatttt
     1201 gaaaagtaag cgaattctat gaaaatgtct aaagaaaatg gggaaacaat ttcaaaaagg
     1261 cacagtttca agtgtttccg aattatacta aatccctcta aaaacttccg gcaaattgat
     1321 atccgtaaaa gagcaaatcc gcatttttgc cgaaaattaa aatttccgac aaatcggcaa
     1381 accggcaatt tggcgaaatt tgccggaagg caattgccgc ccacccctgt tccagaggtt
     1441 caaactggta gcaaagctca aaatttctca aattctccaa tttttttttg aattttggca
     1501 gtgtaccaaa atgacattca gtcatattgg tttattatag atttatttag ataaaatcct
     1561 aaatgattct acctttaaag atgcccactt taaaagtaat gactcaaact tcaaattgct
     1621 ctaagattct attgaattac catcttttcc tctcattttc tctcactgtc tatttcatca
     1681 caaattcatc cctctctccc tctctttctc tctccctctc tctctttctc tttgctcatc
     1741 atctgtcatt ttgtccgttc ctctctctgc gccctcagcg ttccccacac tctctcgctt
     1801 ctcttttcct agacgtcttc ttttttcatc ttcttcagcc ctttttcgcc attttccatc
     1861 tctgtcaatc attacggacg acccccatta atttttggat gtgctctgcg agcaagagca
     1921 cacggaactc atttattttt tgttggaatt tctttttcat tttagttagt taaaaggtta
     1981 ttcagtgcaa aaattgattt tttgctcttt tggtacatta gaggggtttg aaagttttcc
     2041 ataaaaaggg tttccggagt aaaaaatcta aaaattttta caaacatttc caaaaatttt
     2101 gggaacatat cttttcaaaa actcaaaaaa aaacgcaaac atcataaatt taatcaatta
     2161 attggatgcg ttcaaatatt ttaaaaatat ttttgaattg catatttctt tactcataaa
     2221 aaaattatta atgtttaagt aaaaaaaata tttcaggatt ttaaacaaaa actgtaaatc
     2281 ccataaaaca gtgttatttt ttagttattt ttagtaatta tttgtgaatt aaaaacacat
     2341 tttttttcaa aatattcctt gaataaataa aattgaaagt gaattcaaaa tatttcacca
     2401 attttacaaa atttgaaaat ttgaaaaaat attttctacc aaattgtgct caaaaaatat
     2461 tattaagttc ccaggaaatt tatgatgaaa acttggaata tttttggtaa aattttctca
     2521 aaaaattgaa agttgtcaaa aacacactaa cactatacct cgcggcatag aaaaaactgg
     2581 tggccgaatt ttttaaacca atttaaatta aatatatttt tgcactttgt acaaaaaaat
     2641 ttgaagttcc caggaaattc atacggaaat attttccaga acttttataa tttttatttt
     2701 aattctcaaa atttacaata aaaacttgga aacctgaaat ttgcttaaag aataacccta
     2761 tcccccacgg cctagaaaat actggtggcc gaattttttc cgcagccaca ccacctctaa
     2821 tctccagagc actctccaaa attcccataa tccccaaaat tttcagctac aacagcatat
     2881 atgaaggcag agcaacaaca acaatcgatc gcacccggct cggcaacctt cccgtctcag
     2941 gtgaggaaga cgcagagaaa tgagagagag agcgtgttta tgcacgcagt atactaactc
     3001 ctgatatcca atttcatata tcgatatgct tgctttcttt tcttctacgt tgaaattgtt
     3061 tgaacttttc tcaaaaaacg cggattttag agagacttag agttgctcca aataaattta
     3121 atttgcaatt ttgcacttac tattagaatc tacttttata gcgctaaaat gaccgaataa
     3181 cacagtagat tttaaaagaa aattttaaaa attatttttt tgtgcagtca aatttcgcta
     3241 gtttttgcca ctcatttttg aacatttgta tatgtgcctt tcggtggttc aatcaggggt
     3301 ggcacgtggt gtcagagtgt tccatttcgg tttgatctac gttgatctac aaaaaatgcg
     3361 ggagaagaga cgcagagttc tcaactgatt tcacatggtt atgaaccagg ggtgttcggc
     3421 aaatttgccg tttgccgagc tgggcaaatt ttgagatttg ccgcacaccc cttttatgaa
     3481 cgtgctgacg tcacatcttt ttggacaaag aattcccgca ttttttgtat atcaaaccgt
     3541 gatgggacag cctggcacca cgtggggttg gcggcaaatg attttttccg acaaatcggc
     3601 aaattggcaa attgccggaa tttaaagttt ctggcaaatt gttgatttgc cgaaaaaaaa
     3661 tttcaaaaag gcacagtttt aagtgtttcc gtcttataaa atatttctct aaaaatttcc
     3721 ggaaaacgga aaattgccgg aaatacaaag tttcgtcaaa tcggcaaacc tgcaatttgc
     3781 cgaacggcaa ctgccgccaa cacctggttc ccaaaaaagc gtgcactgtt tgaatattaa
     3841 aaaaaaaatt cacacaaaaa taataaattg aaataggcta aattcacatc tatgtacaaa
     3901 aattgcatgc cagcaccggt caattttctc aattactttt tgtctatttt tgccgaacat
     3961 aatttgctgt ttccttggca gaatacagaa aactcttttt tttttcagat gccccgaccc
     4021 ccaccggtaa ccgaacaggc tataaccacc gaggcggagc ttctcgcgaa aaatcaaatt
     4081 acaccaaatg atgtgctagc tcttccagga atcactcaag gtaagtgaat atttgctcta
     4141 ttgataaacg cgccgtgtac tccacgtgga caaacatatc aaattttcag gattcctatg
     4201 ctccccatcg gcaaacgtct ataacattga gttcaccaaa ttccaaatcc gtgacctcga
     4261 cacggaacac gtgctcttcg aaattgcaaa accggaaaat gagacggaag agaatctgca
     4321 ggcgcaagcc gaatcggcaa gatatgtccg atatcgattt gcgccgaatt ttctgaaatt
     4381 aaagacggtc ggcgcgacgg tcgagttcaa ggtcggcgat gtgccgatca cacattttcg
     4441 aatgattgaa cggcactttt tcaaggatcg tttattgaaa tgctttgatt ttgaatttgg
     4501 attctgtatg ccaaattcga gaaacaactg tgaacatatc tatgaatttc cacaactttc
     4561 acaacaactc agtgagtttg aagcgggaaa aattatttga gctcattttg tcaatagagc
     4621 gaattttcta ggtttttaaa aataaaataa atattgacaa aaagctgccc tacagtagtt
     4681 caaaatgtga aaacttggag gaaattcgag ttttctctga gaagtttttg gcgggtaatt
     4741 caaattttct gagaaaaaaa ttagccgcga gaattcaaat tttctgagaa aattatttga
     4801 cgggaaattc aaaatttctg gaaatgtatt gaacatttga gaaattttgt taaaaactac
     4861 attaaaccga taaaaaagtt cgcaaatttt ttttaagtgc tcatggtgca tcgaagagaa
     4921 gaattcttgc caaaactagc ccatgaaatt aaaaattatc gcggaaaaaa actgactaat
     4981 tggaaagtaa aatgaaattt gcaaaaaaaa aatatgtttt tttgttgtaa aattaaaaaa
     5041 aaaactgtat attttcagtg gacgacatga tcaataatcc aaacgagacc cgctccgata
     5101 gcttctattt tgtggagaat aagctcgtaa tgcacaataa ggccgactac tcgtatgatg
     5161 cataatttcc cgccgatgga ataaaactaa taatttttct cgcaatttat agaaatcatg
     5221 tgattttcct accgctaaat ttaaaatttc ctaatttttc actatatttc tcacaaaatg
     5281 cattccgaaa actctcattt ttcagctttt caatacaaat cccaataaat gatttttcaa
     5341 atataatgtt ctcaattgat atgttttatt tgaatgttgg aaaaaaccat aaaattaaaa
     5401 aaaacttgaa ttattcaaaa ataattcggc ccttttacaa aaaattatca taaaaattaa
     5461 atttggaaaa tctgtgattt gtcgctttgc cggaagtttc caattccggc aactaaccga
     5521 tttgccgatt agctagaaat ttcaattccg acaatgtgcc gctttgccga tttgccggaa
     5581 atttccaatt ccggcaatta acggacttgc cgattagcta gaaatttaac tttagataaa
     5641 acttctcaac aaaatcaaat tggaaagtca gcgatttgcc gctttgccgg aagttttcag
     5701 ttccggcatc taaccgattt gccgatttgc caaaaatgtc aattccgaaa aactgccgat
     5761 ttgccgcttt gtcggaaatt tcaattccga caaattgccg atttgccgat ttgccgaaca
     5821 ttccaattcc gaccatttgt cgatttgccg gaagttacca attccggcaa ctaacggatt
     5881 tgccgatttg ccggaaaaaa attgtttgtc gccaaccctg atttaaatga ctaatcatgt
     5941 tgcaaaagat aactgcttgc atcaaaaatc cgatcattgg cattcccaac atcttcttca
     6001 tccaaaacac cacccttttc ttccactaat catgaattcc cgaccaattg ttatcagtag
     6061 gagaagcctc cttttatctc attatcagca attgccttct tcttcttctt ctttgaatac
     6121 catttgaatg gagatatcat ttacataaat ctgccgccga gattgtgaag caggaggaaa
     6181 agctgaagat actgatatta tatgtatatt tcattattct ttttccacaa aattttccga
     6241 catttaattc cacttcactt tcatcgtctc tttttccccc attgtcgccc ccactcattt
     6301 gtcttcttct tcaaatacgg gtcaatcact ttccgttttt tattcatttt ttgttatgtt
     6361 cggcttaaca aatttgaagt attgttaaaa ttctctagag cggccgccac cgcggtggcg
     6421 gccgcgcgga agatctggaa gtaaaatatt gtaatatttt ccctaaattt cacttaaaaa
     6481 taaggatttt tgcaacgtaa aacctcaaaa attcgctgaa attgacgatt tttgttcaaa
     6541 attttgaaaa acagggttaa ttttagctga aaatcttcaa ttttgccaga aattggcttt
     6601 ttttccgtcg ccacacggag aacttgccaa aaatgaagac ttttagccaa tttagccctg
     6661 aaaattcgag ttttgggcga tttttggtct gagaagccgt tttcgcagta tattatattt
     6721 tctcgttaaa tttcatttta aaataaacat tttgaacgta taaccccaaa aattcgctgc
     6781 gcaatggatg atttttgtcc aaagttttga taattttagc taaaaacctc gaattttggc
     6841 aatttttcgt gccgcatcgg ccgaaagttg actttttttg gaattatcat aatttaatgc
     6901 acattttggt agtttatctc acataatctg tctgcaggcg ggaaaaattt gagataaact
     6961 actaaaagat gcaaaacaag gatgataatt ccagaaaatc aacttcggac cgtctcgaca
     7021 cggaaaattg agcgaaattg gagttttaaa atgatttagc cctgaaaatt cgagaaattt
     7081 aggcggtttt gggtctgaaa aggcgttttc gcagtatttt ctcgctaaat ttcatttatt
     7141 atcacataat atcagtactt aggcgaatat cttgtagaaa tgttggtttt gttcgataat
     7201 tctctacttt tagagtcgat tcacgagaaa atgttgcacc atcttacttt tttcccttgt
     7261 aaaacataga ttcttctgta caatgaaaga gaacacaatt tcttcgtgaa gcaactcaaa
     7321 aagtagagaa aattcgaaca aaacccaaca tttctacaag atattcgtct aaatagtgat
     7381 ataatgtgtt aataagcata ttttaaatga aatttagcga aaaaactgcg aaaatttgtc
     7441 aaaaaagcga atttccatcc gatcagcaga tctctaaaag ttacataaaa ttgaaagttt
     7501 tgtggggaaa ttgttttaaa acacgcctat ggtaatacga gaacataaaa ttctgagaat
     7561 gcgtattaca caacatattc gacgcgcaaa atataactat atctcgtagc gaaaactaca
     7621 gtaatcctta aatgactact gtangcgatt gtgtcgattt acgggctcga ttctcggaaa
     7681 aaaaaatcat ttttcaaatt ttgacagcga tattcaattt ttcttcgttt tttcgtatnt
     7741 tctcagtcat ttttgtgctt atttttaata ttttattcat taataaattn aatttaattg
     7801 aaaaacgtcc agacgtattt ttacatttcc cgtctatttc cgtaataggc ggttaataac
     7861 gtagaaatac gtctgtatga gtttactcat caaaatgaca cgaatattgg acgtttttaa
     7921 atcaaattaa tttattaata ggtaaaatat taaaaaataa acacaaaaat gacaaaggaa
     7981 taggaaaaca cgaagagaaa ttgagtatcg ctgtncaaaa ttcgaaaaac gtggattttt
     8041 ccaagaatcg agcccgtaaa tcgacacatt cgctacagta gtcatttaag gattattgta
     8101 gttttcgcta cgagatactt tgcgcgccaa aaacgtggtg aaatacgcat tctcagaatt
     8161 ttatgttccc gtaataccac aatgaccgaa tatcataata aaaaaattca aaaacaattt
     8221 ctagatttta tatgattttc tgaaaattga aaaaatctca gttttcacct aattatattt
     8281 gaactacagc caattgaact cgttcgttgg agcgcgcttg cattattttc attaattaat
     8341 tttattaatc ctcattattt cactgatttt cttcattttt gaggtttttt ttatcgggaa
     8401 atgaaagaaa tatacaagaa aaatgcaaaa tgtttattaa aaactgaaat tagtatttct
     8461 cggagtttta ggcattttca gttacttttt aataaagatt ttgcattttt cttgtatatt
     8521 tctttcattt tccgattaaa aaaccccaaa aatgaagaaa atcagtgaaa taatgaaaat
     8581 taattcaaat aactaatgaa aataatgcaa gcgcgctcca accaacgagt tcaattggcg
     8641 gtaattcaaa tataattagg tgaaaactga gattttttca attttcaaaa aatcatataa
     8701 catctagaca atttttttga atttttttat catgatattc ggtcattgtg tccccatagg
     8761 catgttttaa agcaatttcc acctttaaat aaaatcgaga aaaaatggcg tcaaaagaca
     8821 tatgtaaaag ctgtttctct ttgtatattc acacagaata tagcccgatt ttggaggtga
     8881 aaaaccggtt tttttgtatt ctttacgcca cataaagtga tatggaagag aaaatcgaca
     8941 tatttatgta cttggagtac gagtacattg gagaaaaagg gtgaactact ggaaaatctg
     9001 ggaaatttca gaaaaaaatt ccgaaaaatc ttttttgctg aaaaaaaaat ttgttcgaaa
     9061 tttgggaaat actggaaaat tttcatcaaa attaaagcga tttttaacgt ttttgcttgg
     9121 gagatactat ttgtaatttt aatgtttggt gaggacaaca gaaatgacct tcagcgtacg
     9181 gaaaacgatt aaaagacaca tttgaatcga aaatagcgtg aaaaacatga aaatatcgga
     9241 aaaatagctt taaaattgga tttgaaagca aaaaatgtat caaaaatcga gaaaaatgct
     9301 tgaaaaagtt gaaaaactca aacaaaaaat caaaaaaggt ttttttaaaa atctgtgagt
     9361 atcgcaacga aagcacaact tgaggcatgc gcctttaaac caaccgtaac caaattcgga
     9421 ggcaaaaaaa gatttctcgc tgttttttca gttttaaaat cacttttcac gtttcttttc
     9481 gcagtaattc gtttgcaaaa catctgaaaa aattttattt tataagaaat tatataaaaa
     9541 tcgctataaa ttgagttttt gcccccaaat tcagcacgga gcacttctca attaaatata
     9601 attttatttt ttaacattga aagctttcat aaaattgagc tttgtttgta aataatcagt
     9661 gaaaaaacac agaaatcttc aaaaaataaa gaaaaagttt ttaaaaatct gtgagtcccg
     9721 caacgaaaat aaaactttag gcatgcgcct ttaaaccaac cgtaaccaaa ttcggcggca
     9781 aaaatggatt tctcgccgtt ttttcagttt tacaatcact ttttaggttt ctttttgcag
     9841 tatttcgttt cccaaacaat taaaaatcaa attttctttt ccagatggct caaacaaagc
     9901 cgattgccga gcaaatggga tcccccgggc tgcaggaatt cgatatcaag cttatcgata
     9961 ccgtcgacct cgagggggcg gtaccggtag aaaaaatgag taaaggagaa gaacttttca
    10021 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
    10081 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
    10141 ctactggaaa actacctgtt ccatgggtaa gtttaaacat atatatacta actaaccctg
    10201 attatttaaa ttttcagcca acacttgtca ctactttctg ttatggtgtt caatgcttct
    10261 cgagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt
    10321 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtaagttta
    10381 aacagttcgg tactaactaa ccatacatat ttaaattttc aggtgctgaa gtcaagtttg
    10441 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
    10501 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
    10561 acaaacaaaa gaatggaatc aaagttgtaa gtttaaacat gattttacta actaactaat
    10621 ctgatttaaa ttttcagaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
    10681 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
    10741 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
    10801 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
    10861 aatgcccggg ggatcggtgg agctccaccg gtggcggccg ctctagaact agatcaacaa
    10921 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
    10981 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
    11041 ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
    11101 gattttgagt taggatccgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa
    11161 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
    11221 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
    11281 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
    11341 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
    11401 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
    11461 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
    11521 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
    11581 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
    11641 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
    11701 gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat
    11761 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa gatctggatc
    11821 cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag
    11881 aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtgtgctat gaagcagcgt
    11941 attacagtga cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata
    12001 tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct
    12061 ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct
    12121 cttttgctga cgagaacagg gactggtgaa atgcagttta aggtttacac ctataaaaga
    12181 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga
    12241 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt
    12301 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt
    12361 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc
    12421 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc
    12481 cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
    12541 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
    12601 agctttcttg tacaaagtgg ttgatctagt ggatcctaat tttgccgtat tttccatatt
    12661 ttgttttgta tatttatcca ctcaccccct ctctttgtcc tgtgaatgaa cttgtgccaa
    12721 aaaagccaaa aataatttac tttttttaaa gacatattaa aacttgaaaa cttggaacaa
    12781 tacggtgcga aagcgaaaca gagacagaaa aatgaacaaa gagatccgtg aaaagtgaac
    12841 gatgatttag gcttcttgtt gggcggcgtg gatgagagtg atgttgtctc ctttgagaag
    12901 aattctgccg atcttgttgc gtcccttcgt cttcatgttc acctcctcgg cttcgtcgaa
    12961 cacgacattc atgaactcgt cgaatccgat gatgtagccc tcgagacggt gggtgacatc
    13021 ctcgtacaac cagatttgga cccgagtgcg gttttggaga tatctgaaat ttgggaaatt
    13081 gttttctact gattagtgaa aaaatattac ctgaagatga ggttcacagg ctgaaccatc
    13141 actttgttga gctttctcgt cgacattttt gcgggatgat ctgtaaaaat caatatattt
    13201 tttgaaagac acaaattttt aagataaata tcaagaaaac tcgattttag cagctattta
    13261 agctcaaaaa ctacaattta tggctttaaa acataatttt tcacatttta cataaatttc
    13321 aacgaaaaaa aatgccaaaa aacttcaaat ttgagcagaa atcggctatt ttctcgacaa
    13381 aaataatttc gaaaattcgt tcaaaaaaga tgagtacgca agcaccgtga cgcacagttg
    13441 cggactttgt aaaccgtacc cctgcataaa aaggcggaat tttgaattta ataaaatgca
    13501 gtttagaaca aagaaaagca cggagagaat tgtgataact aattatttgt atttaatttt
    13561 aaatactaca taaaactgaa gattggaacg agttaaagct ggtatttgcg gtttatagcc
    13621 ccaaaagtta aggcttgtta ggcaattatc gataaaaaca acgagaaatt cgtgaaatcg
    13681 gcaaaaattt atagaaaacg tttgaattaa attaaattta cgcttttgcc gccaacacct
    13741 atcgagaccc tagttatagg ggcggagcgt catgggagcg cgcttgcaac tcgatttcaa
    13801 aaagtttcgc tgtttgtaca acattctcag ttgttcttaa tcagttaaat aagtttcaaa
    13861 attttnncaa aatcataaaa cacagttaat gctaaattaa agtctaaaaa natcaggtga
    13921 taaggggttt ggacgaccaa aaagcgacct tcgctcgaaa attgataaac taactacact
    13981 ttttccggaa aaatgatgga aaaatcctga ttttcttcgt tgaatcaatt tttgtttcat
    14041 ttttaacact ttcacatgan naatatttca gttaaaatgc tcaagctaat tgggtttctt
    14101 ataattttgg gtctcgcaac ggccgacaag tgctcagatt gtttggctca ggggaaacag
    14161 tgagttggaa gcttacaatt ctgagaattc gtattgcgca gcttatttga cgcgcaaaat
    14221 atgaaatatc acgtagcgaa aactacagta attctttaaa taagactact gtagtgccta
    14281 tgtcgattta ctgcctcaac tttcttaatc aagctcgtaa atcgacacca gctctacagt
    14341 agtcatagtt ttcgctacga gatattttgc gcgtcaaata tggtgtgcaa tacgcatttt
    14401 cagaatttta tgttctcgta atataccttt tttcagctgg tgtgttactg taaatgcatg
    14461 tggatttacg ccatgtgtca ctacaattac agtgagtttt tttgaagaaa aaaaaattac
    14521 ccatttgatc gccaattcca tttttcaaaa atcaataaaa aaaaaaaatt tcggcgatca
    14581 gaaacgttac agaaagccct aaactgcccg gaactaccag ataaagccca cgcttacgat
    14641 gattcattta tgaggaataa ggtattgatc acacattctg ctgcctacaa tatggaccca
    14701 caaaagtgtt ttacgtaggt tcaccttcta gataaacctt aaaaatcgat actttcagta
    14761 gtaaaatgcc ttcgatgaaa gtttccaaaa cagttaatgt caattgtgat agatatgggc
    14821 caagtgagtt gttttattag cccacggatt tctggcttcc ctcataaatt gaaaaggaag
    14881 agattgccga actagaccat tttggctcgg cgcgttgcgt gtcacgtcgc ggctcgattg
    14941 taaaactaaa tgtattggtc cgtgtggagt acacgacttt ccctttccgg caggcgattg
    15001 tcaatggagc gcgaaaaatt caatgaggaa ggccagaacc ccgtgtctgt agttttctca
    15061 aaaagtggca aaaactcaat aattgccaat ttttgactgc caataaaaat ttcactaaat
    15121 ttattaaatt tattttaagt tttattgaaa tttttttaaa atttccccac tggcgctaaa
    15181 aaatccaatt ttcagaagca atgtgcttca gcttcacggc catagatact gtccaaaaag
    15241 tgattgcaat gacgtttaga gggacagaag gagcagctca attgtctgaa gaaattttgg
    15301 atttcttcca gtaagttcgg gaaatggcaa tttaaagttt aattggattt ttgtattttt
    15361 tcttctcacg actacagttt tgcacatttt tatgttataa aaaaccaaaa aaaaattttt
    15421 ttgccttttt tctcagctta ccacaaaaca atttcgacca aaaataaatt gaaaaaatta
    15481 aaaaattttt tgcacggttt tcggactcaa aaaaagctga atgttcctgg aactatttgt
    15541 aaaattcaaa attcaaaatt ttcacgaatt ctctaaaagg ttccagcaaa ttccacaact
    15601 ttcttgaatt tttcaaaaaa gttcttgagc attcaacaat tatttttgga attttccaaa
    15661 ggttacagga cgttccaatt tttcaaaaaa atttctcgca aaaaatgttt tctcagaaaa
    15721 tttgaatttc cctccacata aaagattgtt ctcaaaaaat ttgcaaacac acattttcag
    15781 cggaaaaaag gcgttcttcg actctggaag tgtattcgaa tatggtaccc agcttttgtt
    15841 ccctttagtg agggttaatt ccgagcttgg cgtaatcatg gtcatagctg tttcctgtgt
    15901 gaaattgtta tccgctcaca attccacaca acataggagc cggaagcata aagtgtaaag
    15961 cctggggtgc ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt
    16021 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    16081 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    16141 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    16201 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    16261 aaaaggccgc gttgctggcg tttttccata ggctcggccc ccctgacgag catcacaaaa
    16321 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgttcc
    16381 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    16441 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
    16501 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    16561 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    16621 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    16681 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
    16741 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    16801 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    16861 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    16921 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    16981 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    17041 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    17101 tagttgcctg actgcccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    17161 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    17221 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    17281 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    17341 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    17401 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgaaaaaaag
    17461 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    17521 tcatgcttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    17581 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    17641 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    17701 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    17761 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    17821 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    17881 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    17941 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    18001 ttccgcgcac atttccccga aaagtgccac ctg
//
