LOCUS       sequence-103930-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway vector for generating green fluorescent protein fusions in 
            fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Gong X, Hurtado O, Wang B, Yi M, Giraldo M, Wu C, Valent B, Goodin 
            MM, Farman M
  TITLE     pFPL vectors for high-throughput protein localization in fungi: 
            detecting cytoplasmic accumulation of putative effector proteins.
  JOURNAL   Mol Plant Microbe Interact. 2014 Nov 12.
  PUBMED    25390188
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2014 Nov 12."
COMMENT     Sequence Label: pFPL-Gh
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     211..232
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    297..421
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(564..583)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc
       61 tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct
      121 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
      181 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
      241 ccaccccggt gaacagctcc tcgcccttgc tcaccatcag gatcccgggc cctatcacca
      301 ctttgtacaa gaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
      361 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
      421 ggtcgacctg cagactggct gtgtataagg gagcctgaca tttatattcc ccagaacatc
      481 aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg
      541 ataacggaga ccggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg
      601 atatgcacca ccgggtaaag ttcacgggag actttatctg acagcagacg tgcactggcc
      661 agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac atccacaaac
      721 agacgataac ggctctctct
//
