LOCUS       sequence-104045-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  epNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in 
            pCS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Tabor KM, Bergeron SA, Horstick EJ, Jordan DC, Aho V, 
            Porkka-Heiskanen T, Haspel G, Burgess HA
  TITLE     Direct activation of the Mauthner cell by electric field pulses 
            drives ultrarapid escape responses.
  JOURNAL   J Neurophysiol. 2014 Aug 15;112(4):834-44. doi: 
            10.1152/jn.00228.2014. Epub 2014 May 21.
  PUBMED    24848468
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/jn.00228.2014"; journalName: "J Neurophysiol"; date: 
            "2014-08-15- 15"; volume: "112"; issue: "4"; pages: "834-44"
COMMENT     Sequence Label: pCS2-epNTR
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        63..81
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     63..80
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    802..936
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(826..845)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     880..899
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctatataagc aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct
       61 tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat ccgccaccat
      121 ggatattatt agtgtggccc tgaagagaca tagcacaaag gcttttgatg ctagcaagaa
      181 gctgacacct gagcaggctg agcagatcaa gacactgctc cagtacagcc ctagcagcct
      241 gaacagccag ccttggcact tcattgtggc tagcacagag gagggaaagg ctagagtggc
      301 taagtctgct gctggaaact atgtgttctc tgagagaaag atgctggatg ctagccatgt
      361 ggtggtgttc tgtgctaaga cagctatgga tgatgtgtgg ctgaagctgg tggtggacca
      421 ggaggatgct gatggaagat ttgctacacc tgaggctaag gctgctaatg acaagggaag
      481 aaagttctgg gctgacatgc acagaaagga cctgcatgat gatgctgagt ggatggctaa
      541 gcaggtgtac ctgaatgtgg gaaacttcct gctgggagtg gctgctctgg gactggatgc
      601 tgtgcctatt gagggatttg atgctgctat cctggatgct gagtttggac tgaaggagaa
      661 gggatacaca agcctggtgg tggtgcctgt gggacaccac tctgtggagg acttcaatgc
      721 tacactgcct aagagcagac tgcctcagaa catcacactg acagaggtgt gatctagaac
      781 tatagtgagt cgtattacgt agatccagac atgataagat acattgatga gtttggacaa
      841 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
      901 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
      961 atgtttcagg ttcaggggga ggtgtggg
//
