LOCUS       sequence-104047-        1118 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  epNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in 
            pCS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Tabor KM, Bergeron SA, Horstick EJ, Jordan DC, Aho V, 
            Porkka-Heiskanen T, Haspel G, Burgess HA
  TITLE     Direct activation of the Mauthner cell by electric field pulses 
            drives ultrarapid escape responses.
  JOURNAL   J Neurophysiol. 2014 Aug 15;112(4):834-44. doi: 
            10.1152/jn.00228.2014. Epub 2014 May 21.
  PUBMED    24848468
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/jn.00228.2014"; journalName: "J Neurophysiol"; date: 
            "2014-08-15- 15"; volume: "112"; issue: "4"; pages: "834-44"
COMMENT     Sequence Label: pCS2-epNTR
FEATURES             Location/Qualifiers
     source          1..1118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     212..229
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    346..367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(382..387,388..405,406..412)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    420..436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     425..447
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     444..460
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     444..460
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     479..499
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        481..499
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    612..746
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(649..668)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     703..722
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
       61 ctccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      121 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      181 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      241 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      301 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      361 cactcattag gcaccccagg ctttacactt tatgctcccg gctcgtatgt tgtgtggaat
      421 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
      481 aattaaccct cactaaaggg aacaaaagct gggtaccggg cccaatgcat tggcgccgcg
      541 gccgcgaatt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
      601 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
      661 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
      721 tcaatgtatc ttatcatgtc tggatctacg taatacgact cactatagtt ctagatcaca
      781 cctctgtcag tgtgatgttc tgaggcagtc tgctcttagg cagtgtagca ttgaagtcct
      841 ccacagagtg gtgtcccaca ggcaccacca ccaggcttgt gtatcccttc tccttcagtc
      901 caaactcagc atccaggata gcagcatcaa atccctcaat aggcacagca tccagtccca
      961 gagcagccac tcccagcagg aagtttccca cattcaggta cacctgctta gccatccact
     1021 cagcatcatc atgcaggtcc tttctgtgca tgtcagccca gaactttctt cccttgtcat
     1081 tagcagcctt agcctcaggt gtagcaaatc ttccatca
//
