LOCUS       sequence-104051-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For 
            ubiquitous genome engineering in Drosophila melanogaster..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Port F, Chen HM, Lee T, Bullock SL
  TITLE     Optimized CRISPR/Cas tools for efficient germline and somatic genome
            engineering in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
  PUBMED    25002478
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Jul 7. pii: 201405500."
COMMENT     Sequence Label: pAct-Cas9
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          87..152
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(251..271)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gatggcattt cttctgagca aaacaggttt tcctcattaa aggcattcca ccactgctcc
       61 cattcatcag ttccataggt tggaatctaa aatacacaaa caattagaat cagtagttta
      121 acacattata cacttaaaaa ttttatattt accttagagc tttaaatctc tgtaggtagt
      181 ttgtccaatt atgtcacacc acagaagtaa ggttccttca caaagatcct ctagaggtac
      241 cctcgagtca caccttcctc ttcttcttgg ggtcagccct gctgtctcca ccgagctgag
      301 agaggtcgat tcttgtttca tagagccccg taattgactg atgaatcagt gtggcgtcca
      361 ggacctcctt tgtagaggtg taccgctttc tgtctatggt ggtgtcgaag tacttgaagg
      421 ctgcaggcgc gcccaagttg gtcagagtaa acaagtggat aatgttttct gcctgctccc
      481 tgatgggctt atccctgtgc ttattgtaag cagaaagcac cttatcgagg ttagcgtcgg
      541 cgaggatcac tcttttggag aattcgctta tttgctcgat gatctcatca aggtagtgtt
      601 tgtgttgttc cacgaacagc tgcttctgct cattatcttc gggagaccct ttgagctttt
      661 catagtggct ggccagatac aagaaattaa cgtatttaga gggcagtgcc agctcgttac
      721 ctttctgcag ctcgcccgca ctagcgagca ttcgtttccg gccgttttca agctcaaaga
      781 gagagtactt gggaagctta atgatgaggt cttttttgac ctctttatat cctttcgcct
      841 cgagaaagtc gatggggttt t
//
