LOCUS       sequence-104052-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For 
            ubiquitous genome engineering in Drosophila melanogaster..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Port F, Chen HM, Lee T, Bullock SL
  TITLE     Optimized CRISPR/Cas tools for efficient germline and somatic genome
            engineering in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
  PUBMED    25002478
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Jul 7. pii: 201405500."
COMMENT     Sequence Label: pAct-Cas9
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttttgccatt tctacattac caaataaggt aaccaaaaac taatgggaaa tccgcattct
       61 ttccattgca gcttacagga tcgatccccg ggaattcacc atggacaaga agtactccat
      121 tgggctcgat atcggcacaa acagcgtcgg ctgggccgtc attacggacg agtacaaggt
      181 gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc cacagcataa agaagaacct
      241 cattggcgcc ctcctgttcg actccgggga gacggccgaa gccacgcggc tcaaaagaac
      301 agcacggcgc agatataccc gcagaaagaa tcggatctgc tacctgcagg agatctttag
      361 taatgagatg gctaaggtgg atgactcttt cttccatagg ctggaggagt cctttttggt
      421 ggaggaggat aaaaagcacg agcgccaccc aatctttggc aatatcgtgg acgaggtggc
      481 gtaccatgaa aagtacccaa ccatatatca tctgaggaag aagcttgtag acagtactga
      541 taaggctgac ttgcggttga tctatctcgc gctggcgcat atgatcaaat ttcggggaca
      601 cttcctcatc gagggggacc tgaacccaga caacagcgat gtcgacaaac tctttatcca
      661 actggttcag acttacaatc agcttttcga agagaacccg atcaacgcat ccggagttga
      721 cgccaaagca atcctgagcg ctaggctgtc caaatcccgg cggctcgaaa acctcatcgc
      781 acagctccct ggggagaaga agaacggcct gtttggtaat cttatcgccc tgtcactcgg
      841 gctgaccccc aactttaaat ctaacttcga cctggccgaa gatgccaagc ttcaactgag
      901 caaagacacc tacgatgatg atctcgacaa tctgctggcc cagatcggcg accagtacgc
      961 agaccttt
//
