LOCUS       sequence-104053-        1101 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR.
            For ubiquitous high specificity genome engineering in Drosophila 
            melanogaster..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1101)
  TITLE     CRISPRflydesign
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAct-Fok1:dCas9
FEATURES             Location/Qualifiers
     source          1..1101
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             104..124
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             131..718
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 ttttgccatt tctacattac caaataaggt aaccaaaaac taatgggaaa tccgcattct
       61 ttccattgca gcttacagga tcgatccccg ggaattcacc atgcctaaga agaagcggaa
      121 ggtgagcagc caacttgtga agtctgaact cgaggagaaa aaatcagagt tgagacacaa
      181 gttgaagtac gtgccacacg aatacatcga gcttatcgag atcgccagaa acagtaccca
      241 ggataggatc cttgagatga aagtcatgga gttctttatg aaggtctacg gttatagagg
      301 aaagcacctt ggcggtagca gaaagcccga tggcgccatc tatactgtcg gatctcctat
      361 cgattatggg gtgatcgtgg ataccaaagc ttactcaggc gggtacaact tgcccatagg
      421 acaagccgac gagatgcagc ggtatgtcga agagaaccag acgcgcaaca agcacatcaa
      481 ccccaatgaa tggtggaaag tgtacccaag tagtgtgact gagttcaagt tcctgtttgt
      541 ctccggccac tttaagggca attataaagc tcagctcact agactcaatc acatcacaaa
      601 ctgcaacgga gctgtgttgt cagtggagga gctcctgatt ggaggcgaga tgatcaaagc
      661 cggcaccctt acactggagg aggtgcggcg gaagttcaac aatggagaga tcaacttcgg
      721 tggcggtgga tccgacaaga agtactccat tgggctcgct atcggcacaa acagcgtcgg
      781 ctgggccgtc attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa
      841 taccgatcgc cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga
      901 gacggccgaa gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa
      961 tcggatctgc tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt
     1021 cttccatagg ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc
     1081 aatctttggc aatatcgtgg a
//
