LOCUS       sequence-104055-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR.
            For ubiquitous high specificity genome engineering in Drosophila 
            melanogaster..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  TITLE     CRISPRflydesign
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAct-Fok1:dCas9
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(107..118)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttagtcagat tatcgaactt ccgttgtgtg atcagtttgg cgttcagcag ctgccgccaa
       61 taatttttca ttttcttgac aacttcttct gaggggacgt tatcactctt ccctctattt
      121 ttatcggatc ttgtcaacac tttattatca atagaatcat ctttgagaaa agactggggc
      181 acgatggcat ccacgtcgta gtcggagagc cgattgatgt ccagttcctg atccacgtac
      241 atgtccctgc cgttctgcag gtagtacagg tagagcttct cattctgaag ctgggtgttt
      301 tcaactgggt gttccttaag gatttgggac cccagttctt ttataccctc ttcaatcctc
      361 ttcatccttt ccctactgtt cttctgtccc ttctgggtag tttggttctc tcgggccatc
      421 tcgataacga tattctcggg cttatgcctt cccattactt tgacgagttc atccacgacc
      481 ttaacggtct gcagtattcc ctttttgata gctgggctac ctgcaagatt agcgatgtgc
      541 tcgtgaagac tgtccccctg gccagaaact tgtgctttct ggatgtcctc cttaaaggtg
      601 agagagtcat catggatcaa ctgcatgaag ttccggttgg caaatccatc ggacttaaga
      661 aaatccagga ttgtctttcc actctgcttg tctcggatcc cattgatcag ttttcttgac
      721 agccgccccc atcctgtata tcggcgcctc ttgagctgtt tcatgacttt gtcgtcgaag
      781 agatgagcgt aagttttcaa gcgttcttca atcatctccc tatcttcaaa caacgtaagg
      841 gtgaggacaa tgtcctcaag aatgtcctcg ttctcctcat tgtccaggaa gtccttgtct
      901 ttaatgattt tcaggagatc gtgatacgtt cccagggatg cgttgaagcg atcctccact
      961 ccgctgattt caacagagtc gaaacattca atctttttga aatagtcttc tttgagctgt
     1021 ttcacggtaa ctttccggtt cgtcttga
//
