LOCUS       sequence-104263-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-ER-5
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             90..800
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 ccgtcagatc cgctagcgcc accatgctgc tatccgtgcc gttgctgctc ggcctcctcg
       61 gcctggccgt cgccgcaccg gtcgccacca tggtgtctaa gggcgaagag ctgattaagg
      121 agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac ttcaagtgca
      181 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg
      241 agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc tacggcagca
      301 agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc ttccctgagg
      361 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg
      421 acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttca
      481 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc accgagacgc
      541 tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg
      601 ggagccatct gatcgcaaac gccaagacca catatagatc caagaaaccc gctaagaacc
      661 tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag gaggccaaca
      721 acgagaccta cgtcgagcag cacgaggtgg cagtggccag atactgcgac ctccctagca
      781 aactggggca caagcttaat tccggaaagg acgagctgta agaattctgc agtcgacggt
      841 accgcgggcc cgggatccac cggatctaga taactgatca taatcagcca taccacattt
      901 gtagaggtt
//
