LOCUS       sequence-104333-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: rsEGFP2-H2B-6
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(125..844)
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNSIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(170..191)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     778..799
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      841..850
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct agagtcgcgg
      121 ccgcttactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
      181 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagcttggac tgatggctca
      241 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
      301 ggtcggcgag ctgcacgctg ccgtcctcaa cgttgtggcg gatcttgaag ttagccttga
      361 tgctgttctt ctgcttgtcg gccgtgatat agacgtagtg gctgttgaag ttgtactcca
      421 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
      481 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtaggta ccgtcgtcct
      541 tgaaagagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
      601 gctgcttcat gtggtcgggg tagcggctga agcaggccac gccgtagccg aaggtggtca
      661 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct
      721 tgccgttggt ggcatcgccc tcgccctcgc cgcggacgct gaacttgtgg ccgtttacgt
      781 cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca
      841 ccatggtggc gaccggtgga tccttagcgc tggtgtactt ggtgatggcc ttagtaccct
      901 cggacacggc gtgcttggcc aactcc
//
