LOCUS       sequence-104362-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MS2 coat protein fused to GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Coulon A, Ferguson ML, de Turris V, Palangat M, Chow CC, Larson DR
  TITLE     Kinetic competition during the transcription cycle results in 
            stochastic RNA processing.
  JOURNAL   Elife. 2014 Oct 1;3. doi: 10.7554/eLife.03939.
  PUBMED    25271374
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-10-1"; volume: "3. doi"; pages: " 10.7554/eLife.03939"
COMMENT     Sequence Label: MS2_GFP
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    113..133
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    540..564
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             573..593
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             597..623
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             597..623
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             624..635
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
       61 gacagcagag atccagtttg gttagtaccg ggcccgctct agattgaatt atcaactttg
      121 tatagaaaag ttggcgacta gtggcctccg cgccgggttt tggcgcctcc cgcgggcgcc
      181 cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct
      241 tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag
      301 tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt
      361 ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct
      421 ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt
      481 ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcactta
      541 caactttgta caaaaaagtt ggcaccatgg gcccaaaaaa gaaaagaaaa gttggctacc
      601 cctacgacgt gcccgactac gccatcgaag gccgccatat gctagccgtt aaaatggctt
      661 ctaactttac tcagttcgtt ctcgtcgaca atggcggaac tggcgacgtg actgtcgccc
      721 caagcaactt cgctaacggg atcgctgaat ggatcagctc taactcgcgt tcacaggctt
      781 acaaagtaac ctgtagcgtt cgtcagagct ctgcgcagaa tcgcaaatac accatcaaag
      841 tcgaggtgcc taaaggcgcc tggcgttcgt acttaaatat ggaactaacc attccaattt
      901 tcgccacgaa ttccgactgc gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg
      961 gaaacccgat tccctcagca atcgcagcaa actccggcat cta
//
